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Optimization of cellulolytic enzyme production by thermophilic fungus <em>Thermoascus aurantiacus</em> using response surface methodology

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Title Statement Optimization of cellulolytic enzyme production by thermophilic fungus <em>Thermoascus aurantiacus</em> using response surface methodology
 
Added Entry - Uncontrolled Name Singh, Anita
Bajar, Somvir
 
Uncontrolled Index Term Biorefineries; Box-Behnken design (BBD); Submerged fermentation; Thermophilic Fungus
 
Summary, etc. In the present study, thermophilic fungus Thermoascus aurantiacus, a local isolate, was used for production of cellulolytic enzymes using rice straw (RS) as substrate under submerged fermentation using Box-Behnken Design (BBD) of Response Surface Methodology (RSM). Themophilic fungus T. aurantiacus grew very well at 50-55°C temperature. BBD was used to study the influence of process parameters, pH (4-6), temperature (40-60°C) and substrate concentration (2-8 g/L) and their interactive effect on cellulase enzyme production. The R2 value 0.94 for Filter paper activity and 0.95 for carboxymethyl cellulase activity indicate that the model is appropriate and replicated to predict the effect of pH, temperature and substrate concentration on both enzyme activities. Under optimized conditions, 6.1 (U/gds) FPase activity and 28.2 (U/gds) CMCase activity was observed.
 
Publication, Distribution, Etc. Indian Journal of Biochemistry and Biophysics (IJBB)
2019-09-25 00:00:00
 
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http://op.niscair.res.in/index.php/IJBB/article/view/28248
 
Data Source Entry Indian Journal of Biochemistry and Biophysics (IJBB); ##issue.vol## 56, ##issue.no## 5 (2019): IJBB Vol.56(5) [October 2019]
 
Language Note en