<strong>Production of biosurfactant from Bacillus subtilis (MF 582633) and its evaluation for antimicrobial, antioxidant, larvicidal and antitermite activities</strong>
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Title Statement |
<strong>Production of biosurfactant from Bacillus subtilis (MF 582633) and its evaluation for antimicrobial, antioxidant, larvicidal and antitermite activities</strong> |
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Added Entry - Uncontrolled Name |
Kanakdande, Amruta P.; Swami Ramanand Teerth Marathwada University , Nanded Khobragade, Chandrahasya N; Swami Ramanand Teerth Marathwada University , Nanded Tabassum, Sana ; Swami Ramanand Teerth Marathwada University , Nanded no funding agency support |
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Uncontrolled Index Term |
Biosurfactant; Biocides; Spectroscopy; Urinary Tract Infection |
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Summary, etc. |
Present study evaluates the production and characterisation of biosurfactant for its antimicrobial, antioxidant, larvicidal and antitermite activities. A bacterium has been isolated from fatty acid contaminated soil and identified as biosurfactant producer , Gram positive <em>Bacillus subtilis </em>(MF582633) subspecies <em>inaquosorum</em> based on 16S rRNA sequencing and unique QR codes. The bacterium produced 2.03 g/L of biosurfactant using 3% glycerol as a carbon source. Among the tested five organisms <em>K.pneumoniae</em> revealed (20.3 mm) and <em>E coli</em> (10 mm) zone of inhibition as compared to standard antibiotic ampicillin (40 µg/mL ) when biosurfactant concentration is reported to be 40 µg/mL. Antioxidant activity in terms of IC<sub>50</sub> value at 0.4 mg/ mL is recorded. The 100% larvicidal activity is recorded at 50 mg/mL concentration of biosurfactant while 40 mg/mL concentration in 48 h revealed 100% antitermite activity. Biosurfactant is purified through silica gel column chromatography. Purified biosurfactant has been characterised using FTIR and <sup>1</sup>H NMR analysis and revealed surfactin type of lipopeptide biosurfactant. Experimental results may add its value in the development of antibiotics and biopesticides to stop the menace of UTI infections, <em>Culex</em> larvae and termites. |
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Publication, Distribution, Etc. |
Indian Journal of Chemical Technology (IJCT) 2019-09-26 15:28:46 |
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Electronic Location and Access |
application/pdf http://op.niscair.res.in/index.php/IJCT/article/view/20648 |
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Data Source Entry |
Indian Journal of Chemical Technology (IJCT); ##issue.vol## 26, ##issue.no## 1 (2019): Indian Journal of Chemical Technololgy |
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Language Note |
en |
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Nonspecific Relationship Entry |
http://op.niscair.res.in/index.php/IJCT/article/download/20648/63524 http://op.niscair.res.in/index.php/IJCT/article/download/20648/63525 http://op.niscair.res.in/index.php/IJCT/article/download/20648/63527 http://op.niscair.res.in/index.php/IJCT/article/download/20648/63528 http://op.niscair.res.in/index.php/IJCT/article/download/20648/63529 http://op.niscair.res.in/index.php/IJCT/article/download/20648/63530 http://op.niscair.res.in/index.php/IJCT/article/download/20648/63531 http://op.niscair.res.in/index.php/IJCT/article/download/20648/63532 http://op.niscair.res.in/index.php/IJCT/article/download/20648/63533 http://op.niscair.res.in/index.php/IJCT/article/download/20648/63534 |
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