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Biodegradation of Anionic surfactant Sodium Dodecyl Sulphate (SDS) and analysis of its metabolic products

Shodhganga@INFLIBNET

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Title Biodegradation of Anionic surfactant Sodium Dodecyl Sulphate (SDS) and analysis of its metabolic products
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Contributor Jisha, M S
 
Subject Biosciences
Microbiology
Anionic surfactant Sodium Dodecyl Sulphat
 
Description ABSTRACT The anionic surfactant Sodium dodecyl sulphate (SDS), the core component of detergent and cosmetic product formulations, contribute significantly to the pollution profile of sewage and wastewaters of all kinds. Due to its high foaming capabilities which can cause numerous problems in sewage treatment facilities as well as the direct toxic effects on many different organisms in the ecosystem, it is generally considered to be a serious pollutant. In this study, 44 SDS degrading strains were isolated by soil enrichment methods and the utilization efficiency was assessed by methylene Blue Active Substances (MBAS) assay and HPLC .The most efficient SDS degrading isolate was selected and identified as Pseudomonas aeruginosa MTCC 10311 based on phenotypic features and 16 S rDNA typing (HM 214777).The selected isolate harbor plasmid but plasmid is not completely responsible for SDS degrading trait. Different physiological characters such as PH, substrate concentration, temperature, incubation time, aeration, carbon sources and nitrogen sources were optimized for efficient SDS degradation. Attempts were made to study the metabolic byproducts of SDS degradation using FTIR and GC-MS analysis. Analysis of ether extracts of surfactant established the sequential production of dodecanol, dodecanal and decanoic acid. At this point, the pathway diverged into the formation of acid residues through beta oxidation. The enzyme responsible for SDS degradation was extracted and purified. The enzyme activity was only 0.0546 U/mg in the crude extract, which on purification by streptomycin sulphate, dialysis, DEAE cellulose and sephadex G200 gave enzyme units of 3.04 U/mg .The partially purified enzyme could give a maximum SDS degradation of 75% at 4 % of enzyme concentration.
newlineThe selected organism was used for the treatment of synthetic wastewater containing SDS. The reduction of SDS and COD in the synthetic wastewater by free cells and immobilized cells of the isolate Pseudomonas aeruginosa were investigated.
Bibliography and Appendix included
 
Date 2014-02-11T04:52:30Z
2014-02-11T04:52:30Z
2014-02-11
n.d.
August 2010
n.d.
 
Type Ph.D.
 
Identifier http://hdl.handle.net/10603/15789
 
Language English US
 
Relation -
 
Rights university
 
Format 160p.
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None
 
Coverage Biosciences
 
Publisher Kottayam
Mahatma Gandhi University
Faculty of Science
 
Source INFLIBNET