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Bioactivity-guided isolation of Laccaic Acid-A: A potent anti-cancer agent from <em>Laccifer lacca</em> (Kerr)

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Title Statement Bioactivity-guided isolation of Laccaic Acid-A: A potent anti-cancer agent from <em>Laccifer lacca</em> (Kerr)
 
Added Entry - Uncontrolled Name Ghosh, Manik
Dey, Suddhasattya
 
Uncontrolled Index Term Laccaic acid-A, Laccifer lacca, MDA-MB-231, Preparative HPLC, SiHa
 
Summary, etc. <em>Laccifer lacca </em>(Kerr) (LL) has widely been used as pigmenting, dying and coloring agent in chemical industry. Although, it has wide range of industrial applications, but unfortunately, due to lesser availability of data, it has been ignored. Keeping in mind, the broad application of <em>Laccifer lacca</em> (Kerr), we tried to report the <em>in vitro</em> anti-cancer effects along with its chemical characterization. This work is divided into two sections, first section to assess the anti-cancer activity of dye of <em>Laccifer lacca </em>(Kerr) employing SRB assay. In second section, the active fraction was processed to isolate the active chemical entities using preparative gradient HPLC-UV. The detector was set at 275 nm and 360 nm as reference wavelength. Results of ethyl acetate fraction (EA) showed promising anti-cancer spectrum for MDA-MB-231 (&lt;10 µg/mL) and SiHa (&lt;10 µg/mL) cell lines. Furthermore, laccaic acid-A was identified from EA of <em>Laccifer lacca</em>. In conclusion, our results suggested being the first report for isolation of laccaic acid-A from <em>Laccifer lacca. </em>We characterized the isolated compound be UV, IR, NMR and Mass spectrometry. We also propose that this is the first report for isolation of laccaic acid-A using preparative HPLC with a good yield and purity
 
Publication, Distribution, Etc. Indian Journal of Traditional Knowledge (IJTK)
2019-10-18 12:46:46
 
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http://op.niscair.res.in/index.php/IJTK/article/view/28998
 
Data Source Entry Indian Journal of Traditional Knowledge (IJTK); ##issue.vol## 18, ##issue.no## 4 (2019): Indian Journal of Traditional Knowledge
 
Language Note en