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Alliance of Efflux Pumps with β-Lactamases in Multidrug-Resistant Isolates.

DIR@IMTECH: CSIR-Institute of Microbial Technology

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Title Alliance of Efflux Pumps with β-Lactamases in Multidrug-Resistant Isolates.
 
Creator Maurya, Navdezda
Jangra, Manoj
Tambat, Rushikesh
Nandanwar, Hemraj
 
Subject QR Microbiology
 
Description Nosocomial infections caused by are primarily characterized by a high prevalence of extended-spectrum β-lactamases (ESBL's) and a soaring pace of carbapenemase dissemination. Availability of limited antimicrobial agents as a therapeutic option for multidrug-resistant bacteria raises an alarming concern. This study aimed at the molecular characterization of multidrug-resistant clinical isolates and studied the role of efflux pumps in β-lactam resistance. Thirty-three isolates confirmed as ESBL-positive that harbored resistance genes to major classes of antibiotics. The results showed that CTX-M15 was the preeminent β-lactamase along with carbapenemases in ESBL-positive isolates. However, the efficacy of different antibiotics varied in the presence of lactamase inhibitors and efflux pump inhibitors (EPIs). Those showing increased efficacy of antibiotics with EPI were further explored for the expression of efflux pump genes and expressed a significantly different level of efflux pumps. We found that an isolate had higher expression of (SMR family) and (MATE family) pump genes relative to RND family pump genes. No mutations were observed in the genes for porins. Together, the findings suggest that β-lactamases are not the only single factor responsible for providing resistance against the existing β-lactam drugs. Resistance may increase many folds by simultaneous expression of RND family (the most prominent family in Gram-negative bacteria) and other efflux pump family.
 
Publisher Mary Ann Liebert
 
Date 2019-10
 
Type Article
PeerReviewed
 
Relation https://www.liebertpub.com/doi/full/10.1089/mdr.2018.0414?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub%3dpubmed
http://crdd.osdd.net/open/2484/
 
Identifier Maurya, Navdezda and Jangra, Manoj and Tambat, Rushikesh and Nandanwar, Hemraj (2019) Alliance of Efflux Pumps with β-Lactamases in Multidrug-Resistant Isolates. Microbial drug resistance (Larchmont, N.Y.), 25 (8). pp. 1155-1163. ISSN 1931-8448