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<p style="text-align: justify;"><strong>Inhibition of pharmacological and toxic effects of <em>Echis </em>carinatus venom by <em>Tabernaemontana </em>alternifolia root extract</strong></p>

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Title Statement <p style="text-align: justify;"><strong>Inhibition of pharmacological and toxic effects of <em>Echis </em>carinatus venom by <em>Tabernaemontana </em>alternifolia root extract</strong></p>
 
Added Entry - Uncontrolled Name M S, Vineetha ; Department of Biochemistry, Centre for Post Graduate Studies, Jain University, Bangalore -560011, India
Janardhan, Bhavya ; Department of Biochemistry, Centre for Post Graduate Studies, Jain University, Bangalore -560011, India
More, Sunil S; School of Basic and Applied Sciences, Dayananda Sagar University, Bangalore-560078, India
DST-SERB Extra mural Grant
 
Uncontrolled Index Term Chick embryo model; Echiscarinatus; Haemorrhage; Procoagulant; Tabernaemontana alternifolia.
 
Summary, etc. <p style="text-align: justify;">The aim of the present study is to validate the anti-venom potential of traditionally used plant <em>Tabernaemontana alternifolia</em> root extract against <em>Echiscarinatus</em> venom by <em>in vitro</em> and <em>in vivo</em> studies. The methanol extract of the plant was evaluated for venom-induced pharmacological activities. Lethal toxicity determination, haemorrhagic studies and its neutralization were studied on chick embryo and mice model.  Oedema and myotoxic neutralization studies were carried out on mice model. At venom:extract 1:60 (w/w) concentrations delayed the clotting time from 12.11 to 100.66 s and at 1:40 (w/w) concentration inhibited the fibrinogen degradation. Further extract significantly inhibited direct and indirect haemolytic activities. The effective dose of extract for 2 LD<sub>50 </sub>of the venom was 0.98 mg/egg. The venom: extract at 1:20(w/w) ratio effectively antagonized venom-induced lethal toxicity. Reference haemorrhagic dose was found to be 2 μg in chick embryo and 10.00 µg was minimum haemorrhagic dose in mice model. Extract completely eliminated the haemorrhage on mice and chick embryo model. Venom-induced oedema ratio of 154% was reduced to 128% and LDH activity from 4520 ± 6.36 to 1685.33 U/L. <em>T. alternifolia</em> possesses highly promising anti-snake venom activity, thus study provided scientific evidence for its traditional use against snakebites.</p>
 
Publication, Distribution, Etc. Indian Journal of Natural Products and Resources (IJNPR) [Formerly Natural Product Radiance (NPR)]
2019-12-18 17:17:26
 
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http://op.niscair.res.in/index.php/IJNPR/article/view/19672
 
Data Source Entry Indian Journal of Natural Products and Resources (IJNPR) [Formerly Natural Product Radiance (NPR)]; ##issue.vol## 10, ##issue.no## 1 (2019): March, 2019
 
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Nonspecific Relationship Entry http://op.niscair.res.in/index.php/IJNPR/article/download/19672/60163
 
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