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Seasonal dynamics of Shatavarin-IV, a potential biomarker of <em>Asparagus racemosus</em> by HPTLC: Possible validation of the ancient Ayurvedic text.

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Title Statement Seasonal dynamics of Shatavarin-IV, a potential biomarker of <em>Asparagus racemosus</em> by HPTLC: Possible validation of the ancient Ayurvedic text.
 
Added Entry - Uncontrolled Name Hazra, Kalyan
Mandal, Achintya Kumar
Mondal, Dhirendra Nath
Ravte, Rohit Kumar
Hazra, Jayram
Rao, Mruthyumjaya Meda
 
Uncontrolled Index Term HPTLC; Quantification; Seasonal; Shatavarin-IV
 
Summary, etc. The medicinal property of <em>Asparagus racemosus </em>is primarily attributed to its constituent steroidal saponins, particularly the major component, shatavarin-IV. Thus, it can serve as a biomarker and its level can decide of the utility of the plant cultivar as a drug. Hence, a sensitive, reliable and quantitative High Performance Thin Layer Chromatography (HPTLC) method has been established for quantification of shatavarin-IV in the methanolic extracts of the roots collected in both summer and rainy seasons. The extracts of the powders of dried roots were applied to silica gel 60 F<sub>254</sub> aluminum-supported precoated TLC plates and developed with n-hexane: ethyl acetate: methanol, 80:10:10 (v/v), as the mobile phase. Shatavarin-IV was detected and quantified by densitometry at λ = 336 nm. The accuracy of the method was checked by conducting recovery studies at three different levels of shatavarin-IV. The average recovery was found to be 101% and 107% for summer and rainy seasons respectively. The shatavarin-IV contents, as estimated by the proposed method were 12.5 μg gm<sup>-1</sup> and 10.9 μg gm<sup>-1</sup> in summer and rainy roots respectively. The entire method was performed six times (n=6) to check the repeatability. The proposed HPTLC method for quantitative monitoring of shatavarin-IV in <em>A. racemosus</em> roots collected in different seasons strictly adhered to the validation issues laid down by the ICH guidelines. The method is reliable reproducible and highly precise and selective.
 
Publication, Distribution, Etc. Indian Journal of Traditional Knowledge (IJTK)
2019-12-27 15:01:53
 
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http://op.niscair.res.in/index.php/IJTK/article/view/30864
 
Data Source Entry Indian Journal of Traditional Knowledge (IJTK); ##issue.vol## 1, ##issue.no## 1 (2020): Indian Journal of Traditional Knowledge
 
Language Note en