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Kelch f-box protein positively influences Arabidopsis seed germination by targeting phytochrome-interacting factor1

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Title Kelch f-box protein positively influences Arabidopsis seed germination by targeting phytochrome-interacting factor1
 
Creator Majee, Manoj
Kumar, Santosh
Kathare, Praveen Kumar
Wu, Shuiqin
Gingerich, Derek
Nayak, Nihar R.
Salaita, Louai
Dinkins, Randy
Martin, Kathleen
Goodin, Michael
Dirk, Lynnette M. A.
Lloyd, Taylor D.
Zhu, Ling
Chappell, Joseph
Hunt, Arthur G.
Vierstra, Richard
Huq, Enamul
Downie, A. Bruce
 
Subject germination
seed
ubiquitination
light
 
Description Accepted date: 15 March 2018
Seeds employ sensory systems that assess various environmental cues over time to maximize the successful transition from embryo to seedling. Here we show that the Arabidopsis F-BOX protein COLD TEMPERATURE-GERMINATING (CTG)-10, identified by activation tagging, is a positive regulator of this process. When overexpressed (OE), CTG10 hastens aspects of seed germination. CTG10 is expressed predominantly in the hypocotyl, and the protein is localized to the nucleus. CTG10 interacts with PHYTOCHROME-INTERACTING FACTOR 1 (PIF1) and helps regulate its abundance in planta. CTG10-OE accelerates the loss of PIF1 in light, increasing germination efficiency, while PIF1-OE lines fail to complete germination in darkness, which is reversed by concurrent CTG10-OE. Double-mutant (pif1 ctg10) lines demonstrated that PIF1 is epistatic to CTG10. Both CTG10 and PIF1 amounts decline during seed germination in the light but reaccumulate in the dark. PIF1 in turn down-regulates CTG10 transcription, suggesting a feedback loop of CTG10/PIF1 control. The genetic, physiological, and biochemical evidence, when taken together, leads us to propose that PIF1 and CTG10 coexist, and even accumulate, in the nucleus in darkness, but that, following illumination, CTG10 assists in reducing PIF1 amounts, thus promoting the completion of seed germination and subsequent seedling development.
Ms. Amy Crume provided the tobacco plants used for
BiFC experiments. Prof. Giltsu Choi (Korea Advanced Institute of Science and
Technology) kindly provided the pil5-1 and PIF1-OE lines. A modified
pRTL2 vector (double CaMV35S promoter, Tobacco etch virus translational
enhancer, NotI sites introduced 5′ and 3′ to the cassette) was the kind gift of
Gulvadee Chaiyaprasithi. Prof. Rup K. Kar (Visva-Bharati University) provided
useful ideas and encouragement at the inception of this project. Dr. Sharyn
Perry (Plant Science Department, University of Kentucky) permitted the use
of her growth chamber, and Dr. Tomokazu Kawashima (Plant Science Department,
University of Kentucky) allowed the use of his imaging system.
Mr. David N. Martin and Mr. Kim Schäfermeyer provided excellent technical
assistance in aspects of the project. Funding support included a pilot project
and research grant from the Kentucky Tobacco Research and Development
Center at the University of Kentucky (to A.B.D.), National Science Foundation
Division of Integrative Organismal Systems Collaborative Research
Grant 0849230 (to A.B.D. and E.H.), NIH Grant 1R01 GM-114297 (to E.H.),
National Science Foundation Supplement 0849230 (to T.D.L. and A.B.D.), an
American Society of Plant Biologists Summer Undergraduate Research Fellowship
(to T.D.L.), US Department of Agriculture–National Institute of Food
and Agriculture Seed Grant 2011-04375 (to A.B.D.), and Kentucky Agricultural
Experiment Station Grant KY011038 (to A.B.D. and L.M.A.D.).
 
Date 2018-04-17T11:19:06Z
2018-04-17T11:19:06Z
2018
 
Type Article
 
Identifier Proceedings of the National Academy of Sciences, USA, 115(17): E4120-E4129
0027-8424
http://223.31.159.10:8080/jspui/handle/123456789/851
http://www.pnas.org/content/early/2018/04/06/1711919115.short?rss=1
https://doi.org/10.1073/pnas.1711919115
 
Language en_US
 
Format application/pdf
 
Publisher PNAS, USA