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Interaction map of Arabidopsis mediator complex expounding its topology

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Title Interaction map of Arabidopsis mediator complex expounding its topology
 
Creator Maji, Sourobh
Dahiya, Pradeep
Waseem, Mohd
Dwivedi, Nidhi
Bhat, Divya S.
Dar, Tanvir H.
Thakur, Jitendra K.
 
Subject Mediator complex
Arabidopsis
topology
Gene Regulation
Chromatin and Epigenetics
 
Description Accepted date: February 20, 2019
Understanding of mechanistic details of Mediator
functioning in plants is impeded as the knowledge of
subunit organization and structure is lacking. In this
study, an interaction map of Arabidopsis Mediator
complex was analyzed to understand the arrangement of the subunits in the core part of the complex. Combining this interaction map with homologybased modeling, probable structural topology of core
part of the Arabidopsis Mediator complex was deduced. Though the overall topology of the complex
was similar to that of yeast, several differences were
observed. Many interactions discovered in this study
are not yet reported in other systems. AtMed14 and
AtMed17 emerged as the key component providing
important scaffold for the whole complex. AtMed6
and AtMed10 were found to be important for linking
head with middle and middle with tail, respectively.
Some Mediator subunits were found to form homodimers and some were found to possess transactivation property. Subcellular localization suggested
that many of the Mediator subunits might have functions beyond the process of transcription. Overall,
this study reveals role of individual subunits in the
organization of the core complex, which can be an
important resource for understanding the molecular
mechanism of functioning of Mediator complex and
its subunits in plants.
We acknowledge Distributed Information Sub-Centre
(DISC) at National Institute of Plant Genome Research
(NIPGR) for computational work. S.M., P.D. and N.D. acknowledge fellowships from University Grants Commission, Government of India, Department of Biotechnology,
Government of India and Council of Scientific and Industrial Research, Government of India, respectively. M.W.,
D.S.B. and T.H.D. acknowledge Short Term Fellowship
from NIPGR. We acknowledge Prof. B. Jayaram of Indian Institute of Technology, New Delhi for critical comments and suggestions. We thank DBT-eLibrary consortium (DeLCON) for all the literatures made available to us. Department of Biotechnology (DBT), Government of India [BT/PR13009/BPA/118/71/2015 and
BT/PR14519/BRB/10/869/2010 to J.K.T.]; Bioinformatics Sub DIC [BT/BI/04/069/2006]. Funding for open
access charge: NIPGR.
 
Date 2019-02-25T07:49:28Z
2019-02-25T07:49:28Z
2019
 
Type Article
 
Identifier Nucleic Acids Research, 47: 3904-3920
1362-4962
http://223.31.159.10:8080/jspui/handle/123456789/921
https://academic.oup.com/nar/advance-article/doi/10.1093/nar/gkz122/5356942
https://doi.org/10.1093/nar/gkz122
 
Language en_US
 
Format application/pdf
 
Publisher Oxford University Press