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Concurrent overexpression of rice G-protein β and γ subunits provide enhanced tolerance to sheath blight disease and abiotic stress in rice

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Title Concurrent overexpression of rice G-protein β and γ subunits provide enhanced tolerance to sheath blight disease and abiotic stress in rice
 
Creator Swain, Durga Madhab
Sahoo, Ranjan Kumar
Chandan, Ravindra Kumar
Ghosh, Srayan
Kumar, Rahul
Jha, Gopaljee
Tuteja, Narendra
 
Subject Antioxidants
Biotic stress
Defense marker genes
Drought stress
G-protein
MAP kinase
Overexpression
ROS
R. solani
Salinity stress
 
Description Accepted date: 15 July 2019
The heterotrimeric G-proteins act as signalling molecules and modulate various cellular responses including stress
tolerance in eukaryotes. The gamma (γ) subunit of rice G-protein (RGG1) was earlier reported to promote salinity stress
tolerance in rice. In the present study, we report that a rice gene-encoding beta (β) subunit of G-protein (RGB1) gets upregulated during both biotic (upon a necrotrophic fungal pathogen, Rhizoctonia solani infection) and drought stresses. Markerfree transgenic IR64 rice lines that simultaneously overexpress both RGB1 and RGG1 genes under CaMV35S promoter
were raised. The overexpressing (OE) lines showed enhanced tolerance to R. solani infection and salinity/drought stresses.
Several defense marker genes including OsMPK3 were signifcantly upregulated in the R. solani-infected OE lines. We also
found the antioxidant machineries to be upregulated during salinity as well as drought stress in the OE lines. Overall, the
present study provides evidence that concurrent overexpression of G-protein subunits (RGG1 and RGB1) impart multiple
(both biotic and abiotic) stress tolerance in rice which could be due to the enhanced expression of stress-marker genes and
better management of reactive oxygen species (ROS)-scavenging/photosynthetic machinery. The current study suggests an
improved approach for simultaneous improvement of biotic and abiotic stress tolerance in rice which remains a major challenge for its sustainable cultivation.
DMS acknowledges post-doctoral fellowship from
Department of Biotechnology (DBT), Govt. of India. Work on abiotic
stress tolerance in NT laboratory is supported by the DBT, Government of India. SG acknowledges SPM fellowship from CSIR, India.
RK acknowledges SRA fellowship from CSIR, India. Work in GJ lab
was supported by core research grant from National Institute of Plant
Genome Research, India and research funding from DBT, Govt. of
India.
 
Date 2019-07-26T07:45:30Z
2019-07-26T07:45:30Z
2019
 
Type Article
 
Identifier Planta, 250(5): 1505-1520
1432-2048
http://223.31.159.10:8080/jspui/handle/123456789/964
https://link.springer.com/article/10.1007%2Fs00425-019-03241-z
https://doi.org/10.1007/s00425-019-03241-z
 
Language en_US
 
Format application/pdf
 
Publisher Springer Nature Publishing AG