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Arabidopsis SKP1-like protein 13 (ASK13) positively regulates seed germination and seedling growth under abiotic stresses

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Title Arabidopsis SKP1-like protein 13 (ASK13) positively regulates seed germination and seedling growth under abiotic stresses
 
Creator Rao, Venkateswara
Petla, Bhanu Prakash
Verma, Pooja
Salvi, Prafull
Kamble, Nitin Uttam
Ghosh, Sharboni
Kaur, Harmeet
Saxena, Saurabh C
Majee, Manoj
 
Subject ASK13
abiotic stress
F-box proteins
germination
protein interaction
proteasome
SCF complex
seedling growth
seed vigor
 
Description Accepted date: 14 May 2018
SKP1 (S-Phase Kinase Associated Protein1) proteins are key members of the SCF (SKP-Cullin-F-box protein) E3 ligase complexes that ubiquitinate the target proteins and play diverse roles in plant biology. However, as compared to other members of the SCF complex, study of SKP1-like proteins is very limited in plants. In the present work, we report that Arabidopsis SKP1-like protein13 (ASK13) is differentially regulated in different organs, during seed development and germination, and is upregulated in response to abiotic stresses. Y2H library screening and subsequent in vivo interaction through BiFC analysis revealed that ASK13 not only interacts with F-box proteins but also with other proteins which are not components of SCF complexes. Biochemical analysis revealed that ASK13 not only exists as a monomer but also as a homo-oligomer or heteromer with other ASK proteins. Functional analysis using ASK13 overexpression and knockdown lines revealed that ASK13 positively influences seed germination and seedling growth particularly under abiotic stresses. Taken together, our data strongly suggests that apart from participation to form SCF complexes, ASK13 interacts with several other proteins and is implicated in different cellular processes distinct from protein degradation. Overall, ASK13 positively regulates seed germination and seedling growth particularly under abiotic stress conditions.
This work was supported by the NIPGR core grant; Department of Biotechnology, Government
of India V.R, P.S, N.K and SG thank Council of Scientific and Industrial Research and University
Grant Commission, Government of India, for research fellowships.
 
Date 2018-05-22T11:22:24Z
2018-05-22T11:22:24Z
2018
 
Type Article
 
Identifier Journal of Experimental Botany, 69(16): 3899-3915
1460-2431
http://223.31.159.10:8080/jspui/handle/123456789/858
https://academic.oup.com/jxb/advance-article/doi/10.1093/jxb/ery191/4999180
https://doi.org/10.1093/jxb/ery191
 
Language en_US
 
Format application/pdf
 
Publisher Oxford University Press