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Heterotic patterns of primary and secondary metabolites in the oilseed crop Brassica juncea

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Title Heterotic patterns of primary and secondary metabolites in the oilseed crop Brassica juncea
 
Creator Bajpai, Prabodh K.
Reichelt, Michael
Augustine, Rehna
Gershenzon, Jonathan
Bisht, Naveen C.
 
Subject Brassica juncea
oilseed crop
primary and secondary metabolites
Heterotic patterns
 
Description Accepted date: 11 March 2019
Heterosis refers to the superior performance of F1 hybrids over their respective parental inbred lines. Although the genetic
and expression basis of heterosis have been previously investigated, the metabolic basis for this phenomenon is poorly
understood. In a preliminary morphological study in Brassica juncea, we observed significant heterosis at the 50% flowering
stage, wherein both the growth and reproduction of F1 reciprocal hybrids were greater than that of their parents. To identify
the possible metabolic causes or consequences of this heterosis, we carried out targeted LC-MS analysis of 48 primary
(amino acids and sugars) and secondary metabolites (phytohormones, glucosinolates, flavonoids, and phenolic esters) in five
developmental tissues at 50% flowering in hybrids and inbred parents. Principal component analysis (PCA) of metabolites
clearly separated inbred lines from their hybrids, particularly in the bud tissues. In general, secondary metabolites displayed
more negative heterosis values in comparison to primary metabolites. The tested primary and secondary metabolites
displayed both additive and non-additive modes of inheritance in F1 hybrids, wherein the number of metabolites showing an
additive mode of inheritance were higher in buds and siliques (52.77–97.14%) compared to leaf tissues (47.37–80%). Partial
least regression (PLS) analysis further showed that primary metabolites, in general, displayed higher association with
morphological parameters in F1 hybrids. Overall, our results are consistent with a resource-cost model for heterosis in B.
juncea, where metabolite allocation in hybrids appears to favor growth, at the expense of secondary metabolism.
The work was supported by NIPGR core grant to
NCB. PKB acknowledge the National Post-doctoral fellowship (NPDF
grant no. PDF/2015/000391/LS) from SERB, DST (India). RA was
funded with short-term research fellowship from NIPGR, India. NCB
acknowledges the Max Planck-India mobility grant jointly funded by
Max Planck Society (Germany) and DST (India). MR and JG
acknowledge the funding by the Max Planck Society (Germany). We
sincerely thank Dr. Payal Sanadhya (PS) for her help in drawing Fig. 4
and Dr. Arun Jagannath for language editing. Insightful suggestions
from two anonymous reviewers are highly acknowledged.
 
Date 2019-03-28T06:48:16Z
2019-03-28T06:48:16Z
2019
 
Type Article
 
Identifier Heredity, 123(3): 318-336
1365-2540
http://223.31.159.10:8080/jspui/handle/123456789/935
https://www.nature.com/articles/s41437-019-0213-3
https://doi.org/10.1038/s41437-019-0213-3
 
Language en_US
 
Format application/pdf
 
Publisher Springer Nature