Development of efficient protocol for rice transformation overexpressing MAP kinase and their effect on root phenotypic traits
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Title |
Development of efficient protocol for rice transformation overexpressing MAP kinase and their effect on root phenotypic traits
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Creator |
Singh, Pallavi
Ara, Hussain Tayyeba, Sumaira Pandey, Chandana Sinha, Alok Krishna |
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Subject |
Rice
Transgenic MAPK Indica Japonica Promoter RSA Dexamethasone GiA Roots |
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Description |
Accepted date: 15 February 2019
Exhaustive studies on mitogen-activated protein kinase (MAPK) have reported the importance in regulating a variety of responses during plant growth and development. In particular, the potential MAPK genes, MPK3 and MPK6, seem to regulate a plethora of responses, conferring tolerance to varied abiotic, biotic, and developmental stimuli. This makes both MPK3 and MPK6 potential targets for further studies. It would be an important concern to overexpress and knock out these pivotal proteins and then, in turn, to monitor the plant response which is expected to correlate action of a gene to a trait in cellular and organismal contexts. However, overexpression of MAPK genes has remained a puzzle in plants. In the present study, we report the generation of stable transgenic lines overexpressing OsMPK3 in indica and japonica cultivars and OsMPK6 in japonica cultivar under the control of an inducible promoter. We also establish the crucial steps and troubleshooting for each of the indicated rice transformation medium components. Later, we study the potential role of these MAPKs in high-throughput analysis of root system architectural (RSA) traits. It was observed that OsMPK6 overexpression lines had a more robust and spread out root architectural system while OsMPK3 overexpression lines had a typical bushy phenotype. The authors received financial assistance from the National Institute of Plant Genome Research (NIPGR), Department of Biotechnology (DBT), Government of India. HA received fellowship from the Department of Science and Technology-Women Scientist Scheme (DST-WOS); PS and ST, Council of Scientific Research (CSIR), Government of India; CP, Science and Engineering Research Board for National Postdoctoral Fellowship. The authors are thankful to DBT-eLibrary Consortium (DeLCON) for providing access to e-resources. |
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Date |
2019-02-28T05:53:04Z
2019-02-28T05:53:04Z 2019 |
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Type |
Article
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Identifier |
Protoplasma, 256(4): 997-1011
1615-6102 http://223.31.159.10:8080/jspui/handle/123456789/923 https://link.springer.com/article/10.1007/s00709-019-01359-1 https://doi.org/10.1007/s00709-019-01359-1 |
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Language |
en_US
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Format |
application/pdf
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Publisher |
Springer Nature
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