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Development of efficient protocol for rice transformation overexpressing MAP kinase and their effect on root phenotypic traits

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Title Development of efficient protocol for rice transformation overexpressing MAP kinase and their effect on root phenotypic traits
 
Creator Singh, Pallavi
Ara, Hussain
Tayyeba, Sumaira
Pandey, Chandana
Sinha, Alok Krishna
 
Subject Rice
Transgenic
MAPK
Indica
Japonica
Promoter
RSA
Dexamethasone
GiA Roots
 
Description Accepted date: 15 February 2019
Exhaustive studies on mitogen-activated protein kinase (MAPK) have reported the importance in regulating a variety of
responses during plant growth and development. In particular, the potential MAPK genes, MPK3 and MPK6, seem to
regulate a plethora of responses, conferring tolerance to varied abiotic, biotic, and developmental stimuli. This makes
both MPK3 and MPK6 potential targets for further studies. It would be an important concern to overexpress and knock
out these pivotal proteins and then, in turn, to monitor the plant response which is expected to correlate action of a gene
to a trait in cellular and organismal contexts. However, overexpression of MAPK genes has remained a puzzle in plants.
In the present study, we report the generation of stable transgenic lines overexpressing OsMPK3 in indica and japonica
cultivars and OsMPK6 in japonica cultivar under the control of an inducible promoter. We also establish the crucial steps
and troubleshooting for each of the indicated rice transformation medium components. Later, we study the potential role
of these MAPKs in high-throughput analysis of root system architectural (RSA) traits. It was observed that OsMPK6
overexpression lines had a more robust and spread out root architectural system while OsMPK3 overexpression lines had
a typical bushy phenotype.
The authors received financial assistance from the
National Institute of Plant Genome Research (NIPGR), Department of
Biotechnology (DBT), Government of India. HA received fellowship
from the Department of Science and Technology-Women Scientist
Scheme (DST-WOS); PS and ST, Council of Scientific Research
(CSIR), Government of India; CP, Science and Engineering Research
Board for National Postdoctoral Fellowship. The authors are thankful to DBT-eLibrary
Consortium (DeLCON) for providing access to e-resources.
 
Date 2019-02-28T05:53:04Z
2019-02-28T05:53:04Z
2019
 
Type Article
 
Identifier Protoplasma, 256(4): 997-1011
1615-6102
http://223.31.159.10:8080/jspui/handle/123456789/923
https://link.springer.com/article/10.1007/s00709-019-01359-1
https://doi.org/10.1007/s00709-019-01359-1
 
Language en_US
 
Format application/pdf
 
Publisher Springer Nature