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Neomycin: an effective inhibitor of jasmonate-induced reactions in plants

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Title Neomycin: an effective inhibitor of jasmonate-induced reactions in plants
 
Creator Vadassery, Jyothilakshmi
Ballhorn, Daniel J.
Fleming, Steven R.
Mazars, Christian
Pandey, Shree P.
Schmidt, Axel
Schuman, Meredith C.
Yeh, Kai‑Wun
Yilamujiang, Ayufu
Mithöfer, Axel
 
Subject Jasmonate
Inhibitor
Defense
Dicots
Gymnosperms
Monocots
Neomycin
Signaling
 
Description Accepted date: 11 October 2018
Jasmonates are important phytohormones involved in both plant developmental processes as well as defense reactions. Many
JA-mediated plant defense responses have been studied in model plants using mutants of the jasmonate signaling pathway.
However, in plant species where JA-signaling mutants are not accessible, the availability of a tool targeting JA signaling is
crucial to investigate jasmonate-dependent processes. Neomycin is a poly-cationic aminoglycoside antibiotic that blocks the
release of Ca2+ from internal stores. We examined the inhibitory activities of neomycin on different jasmonate-inducible
responses in eight different plant species: Intracellular calcium measurements in Nicotiana tabacum cell culture, Sporamin
gene induction in Ipomoea batatas, PDF2.2 gene expression in Triticum aestivum, Nepenthesin protease activity measurement
in Nepenthes alata, extrafloral nectar production in Phaseolus lunatus, nectary formation in Populus trichocarpa, terpene
accumulation in Picea abies, and secondary metabolite generation in Nicotiana attenuata. We are able to show that neomycin,
an easily manageable and commercially available compound, inhibits JA-mediated responses across the plant kingdom.
We thank the MPI-CE greenhouse team for plant
cultivation. It is gratefully acknowledged that this work was supported
by the Max Planck Society, by MPG-India partner group program of the Max Planck Society (Germany) and Department of Science and
Technology, India for JV, by the German Academic Exchange Service
(DAAD; PPP Project ID 57136171) to AM, by Funding of the National
Science Foundation (NSF) (Grants IOS 1457369 and 1656057) to DJB.
 
Date 2018-11-15T07:43:40Z
2018-11-15T07:43:40Z
2019
 
Type Article
 
Identifier Journal of Plant Growth Regulation, 38(2): 713-722
1435-8107
http://223.31.159.10:8080/jspui/handle/123456789/896
https://link.springer.com/article/10.1007/s00344-018-9884-x?wt_mc=Internal.Event.1.SEM.ArticleAuthorOnlineFirst&utm_source=ArticleAuthorOnlineFirst&utm_medium=email&utm_content=AA_en_06082018&ArticleAuthorOnlineFirst_20181112
https://doi.org/10.1007/s00344-018-9884-x
 
Language en_US
 
Format application/pdf
 
Publisher Springer Nature