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Differential regulation of genes involved in root morphogenesis and cell wall modification is associated with salinity tolerance in chickpea

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Title Differential regulation of genes involved in root morphogenesis and cell wall modification is associated with salinity tolerance in chickpea
 
Creator Kaashyap, Mayank
Ford, Rebecca
Kudapa, Himabindu
Jain, Mukesh
Edwards, Dave
Varshney, Rajeev
Mantri, Nitin
 
Subject Gene expression
Salt
Root Morphogenesis
Chickpea
Salinity Tolerance
 
Description Accepted date: 06 March 2018
Salinity is a major constraint for intrinsically salt sensitive grain legume chickpea. Chickpea exhibits large genetic variation amongst cultivars, which show better yields in saline conditions but still need to be improved further for sustainable crop production. Based on previous multi-location physiological screening, JG 11 (salt tolerant) and ICCV 2 (salt sensitive) were subjected to salt stress to evaluate their physiological and transcriptional responses. A total of ~480 million RNA-Seq reads were sequenced from root tissues which resulted in identification of 3,053 differentially expressed genes (DEGs) in response to salt stress. Reproductive stage shows high number of DEGs suggesting major transcriptional reorganization in response to salt to enable tolerance. Importantly, cationic peroxidase, Aspartic ase, NRT1/PTR, phosphatidylinositol phosphate kinase, DREB1E and ERF genes were significantly up-regulated in tolerant genotype. In addition, we identified a suite of important genes involved in cell wall modification and root morphogenesis such as dirigent proteins, expansin and casparian strip membrane proteins that could potentially confer salt tolerance. Further, phytohormonal cross-talk between ERF and PIN-FORMED genes which modulate the root growth was observed. The gene set enrichment analysis and functional annotation of these genes suggests they may be utilised as potential candidates for improving chickpea salt tolerance.
Authors are also grateful to VLSCI (Melbourne Bioinformatics) for providing some of the bioinformatics tools
used for analysis. Tis work was fnancially supported by Australia-India Strategic Research Fund (AISRF) Grant
number GCF010013, Commonwealth of Australia.
 
Date 2018-03-23T05:11:56Z
2018-03-23T05:11:56Z
2018
 
Type Article
 
Identifier Scientific Reports, 8(1): 4855
2045-2322
http://223.31.159.10:8080/jspui/handle/123456789/841
https://www.nature.com/articles/s41598-018-23116-9
10.1038/s41598-018-23116-9
 
Language en_US
 
Format application/pdf
 
Publisher Nature Publishing Group