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Nitric oxide accelerates germination via the regulation of respiration in chickpea

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Title Nitric oxide accelerates germination via the regulation of respiration in chickpea
 
Creator Pandey, Sonika
Kumari, Aprajita
Shree, Manu
Kumar, Vinod
Singh, Pooja
Bharadwaj, Chellapilla
Loake, Gary J.
Parida, Swarup K.
Masakapalli, Shyam Kumar
Gupta, Kapuganti Jagadis
 
Subject Alternative oxidase
hydrogen peroxide
nitric oxide
nitrite
reactive oxygen species
superoxide
 
Description Accepted date: 11 April 2019
Seed germination is crucial for the plant life cycle. We investigated the role of nitric oxide (NO) in two chickpea varieties that differ in germination capacity: Kabuli, which has a low rate of germination and germinates slowly, and Desi,
which shows improved germination properties. Desi produced more NO than Kabuli and had lower respiratory rates.
As a result of the high respiration rates, Kabuli had higher levels of reactive oxygen species (ROS). Treatment with
the NO donor S-nitroso-N-acetyl-D,L-penicillamine (SNAP) reduced respiration in Kabuli and decreased ROS levels,
resulting in accelerated germination rates. These findings suggest that NO plays a key role in the germination of
Kabuli. SNAP increased the levels of transcripts encoding enzymes involved in carbohydrate metabolism and the cell
cycle. Moreover, the levels of amino acids and organic acids were increased in Kabuli as a result of SNAP treatment.
1
H-nuclear magnetic resonance analysis revealed that Kabuli has a higher capacity for glucose oxidation than Desi.
An observed SNAP-induced increase in 13C incorporation into soluble alanine may result from enhanced oxidation
of exogenous [13C]glucose via glycolysis and the pentose phosphate pathway. A homozygous hybrid that originated
from a recombinant inbred line population of a cross between Desi and Kabuli germinated faster and had increased
NO levels and a reduced accumulation of ROS compared with Kabuli. Taken together, these findings demonstrate the
importance of NO in chickpea germination via the control of respiration and ROS accumulation.
KJG is funded by IYBA and a Ramalingaswami fellowship, Indo-Swiss
joint research program from Department of Biotechnology, Govt of
India. KJG is also supported by an SERB-ECR award. SP is the recipient of a National Postdoctoral Fellowship from SERB, Department
of Science and Technology, Govt of India. PS is the recipient of a JRF
and SRF fellowship from UGC, India. We thank Prof. Abir Igamberdiev
for critical reading of the manuscript and valuable suggestions. We
thank Dr Manish Kumar Patel for his valuable help in measuring metabolites and lipids.
 
Date 2019-06-06T10:14:28Z
2019-06-06T10:14:28Z
2019
 
Type Article
 
Identifier Journal of Experimental Botany, 70(17): 4539-4555
1460-2431
http://223.31.159.10:8080/jspui/handle/123456789/955
https://academic.oup.com/jxb/advance-article/doi/10.1093/jxb/erz185/5479413
https://doi.org/10.1093/jxb/erz185
 
Language en_US
 
Format application/pdf
 
Publisher Oxford University Press