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KIX domain of AtMed15a, a Mediator subunit of Arabidopsis, is required for its interaction with different proteins

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Title KIX domain of AtMed15a, a Mediator subunit of Arabidopsis, is required for its interaction with different proteins
 
Creator Kumar, Vinay
Waseem, Mohd
Dwivedi, Nidhi
Maji, Sourobh
Kumar, Angad
Thakur, Jitendra K.
 
Subject Mediator
Med15
KIX domain
transcription factors
transactivation
 
Description Accepted date: 26 December 2017
Med15 is an important subunit of Mediator tail module and is characterized by a KIX domain present in the amino terminal. In yeast and metazoans, Med15 KIX domain has been found to interact with various transcription factors, regulating several processes including carbohydrate metabolism, lipogenesis, stress response and multidrug resistance. Mechanism of Med15 functioning in Arabidopsis is largely unknown. In this study, interactome of Arabidopsis Med15, AtMed15a, was characterized. We found 45 proteins that interact with AtMed15a KIX domain, including 11 transcription factors, 3 single strand nucleic acid-binding proteins and 1 splicing factor. The third helix of the KIX domain was found to be involved in most of the interactions. Mapping of the regions participating in the interactions revealed that the activation domain of a transcription factor, UKTF1 interacted with AtMed15a KIX domain. Thus, our results suggest that in Arabidopsis, activation domain of transcription factors target KIX domain of AtMed15a for their transcriptional responses.
This work was supported by a grant (EMR/2015/001336) funded by Science and Engineering
Board, Department of Science and Technology, Government of India. We wish to thank UGC
for granting Senior Research Fellowships to VK, SM and AK; CSIR for granting Senior
Research Fellowship to ND, and NIPGR for granting Senior Research Fellowship to MW.
 
Date 2018-01-22T09:05:54Z
2018-01-22T09:05:54Z
2018
 
Type Article
 
Identifier Plant Signaling & Behavior, 13(2): e1428514
1559-2324
http://223.31.159.10:8080/jspui/handle/123456789/825
http://www.tandfonline.com/doi/full/10.1080/15592324.2018.1428514
https://doi.org/10.1080/15592324.2018.1428514
 
Language en
 
Format application/pdf
 
Publisher Taylor & Francis Group