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Molecular basis of the evolution of methylthioalkylmalate synthase and diversity of methionine-derived glucosinolates

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Title Molecular basis of the evolution of methylthioalkylmalate synthase and diversity of methionine-derived glucosinolates
 
Creator Kumar, Roshan
Lee, Soon Goo
Augustine, Rehna
Reichelt, Micheal
Vassão, Daniel G.
Palavalli, Manoj H.
Allen, Aron
Gershenzon, Jonathan
Jez, Joseph M.
Bisht, Naveen C.
 
Subject Glucosinolates
Methylthioalkylmalate Synthase
 
Description Accepted date: April 19, 2019
Methylthioalkylmalate synthase catalyzes the committed step in the side-chain elongation of methionine-derived aliphatic glucosinolates and likely evolved from the isopropylmalate synthases of leucine biosynthesis. The globally cultivated Brassica species possess diverse aliphatic glucosinolates important for plant defense and animal nutrition; however, the molecular basis for the evolution of methylthioalkylmalate synthase and its generation of natural product diversity in Brassica is poorly understood. Here we show that Brassica genomes encode multiple methylthioalkylmalate synthase that have differences in expression profiles and 2-oxo substrate preference that account for diversity of aliphatic glucosinolates across Brassica accessions. The 2.1 Å resolution x-ray crystal structure of B. juncea methylthioalkylmalate synthase identifies key active site residues responsible for controlling specificity for different 2-oxo substrates and the determinants of side-chain length in aliphatic glucosinolates. Overall, these results provide the evolutionary and biochemical foundation for diversification of glucosinolates profiles across globally-cultivated Brassica species, which could be used with ongoing breeding strategies towards manipulation of beneficial glucosinolates compounds for animal health and plant protection.
The work was supported by grants BT/PR271/AGR/36/687/2011 and BT/06/IYBA/2012 of the
Department of Biotechnology, India to N.C.B.; a National Science Foundation grant to J.M.J. (NSF-MCB-1614539), and the Max Planck Society to J.G. N.C.B. acknowledges Max Planck India
Fellowship and NIPGR-Short Term Ovearseas Fellowship. R.K. and R.A. acknowledge financial
support from UGC (India) and NIPGR, respectively. We are grateful to Central Instrumentation and
Plant Growth Facilities at NIPGR. Portions of this research were carried out at the Argonne National
Laboratory Structural Biology Center of the Advanced Photon Source, a national use facility operated
by the University of Chicago for the Department of Energy Office of Biological and Environmental
Research under Grant DE-AC02-06CH11357.
 
Date 2019-04-30T07:55:29Z
2019-04-30T07:55:29Z
2019
 
Type Article
 
Identifier Plant Cell, 31(7): 1633-1647
1531-298X
http://223.31.159.10:8080/jspui/handle/123456789/946
http://www.plantcell.org/content/early/2019/04/25/tpc.19.00046.long
https://doi.org/10.1105/tpc.19.00046
 
Language en_US
 
Format application/pdf
 
Publisher American Society of Plant Biologists