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Xanthomonas oryzae pv. oryzae XopQ protein suppresses rice immune responses through interaction with two 14-3-3 proteins but its phospho-null mutant induces rice immune responses and interacts with another 14-3-3 protein

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Title Xanthomonas oryzae pv. oryzae XopQ protein suppresses rice immune responses through interaction with two 14-3-3 proteins but its phospho-null mutant induces rice immune responses and interacts with another 14-3-3 protein
 
Creator Deb, Sohini
Gupta, Mahesh K.
Patel, Hitendra K.
Sonti, Ramesh V.
 
Subject Xanthomonas oryzae pv. oryzae
14-3-3 protein
XopQ
resistance
rice
effector
 
Description Accepted date: 15 May 2019
Many bacterial phytopathogens employ effectors secreted through the type-III secretion system to suppress plant innate immune responses. The Xanthomonas type-III secreted non-TAL effector protein Xanthomonas outer protein Q (XopQ) exhibits homology to nucleoside hydrolases. Previous work indicated that mutations which affect the biochemical activity of XopQ fail to affect its ability to suppress rice innate immune responses, suggesting that the effector might be acting through some other pathway or mechanism. In this study, we show that XopQ interacts in yeast and in planta with two rice 14-3-3 proteins, Gf14f and Gf14g. A serine to alanine mutation (S65A) of a 14-3-3 interaction motif in XopQ abolishes the ability of XopQ to interact with the two 14-3-3 proteins and to suppress innate immunity. Surprisingly, the S65A mutant gains the ability to interact with a third 14-3-3 protein that is a negative regulator of innate immunity. The XopQS65A mutant is an inducer of rice immune responses and this property is dominant over the wild-type function of XopQ. Taken together, these results suggest that XopQ targets the rice 14-3-3 mediated immune response pathway and that its differential phosphorylation might enable interaction with alternative 14-3-3 proteins.
We acknowledge Mary B. Mudgett (Stanford University) for
providing the BiFC vectors pDEST-VYNE(R)GW and pDESTVYNE(R)GW. This work was supported by grants to RVS
from the Plant-Microbe and Soil Interaction (PMSI) project
of the Council of Scientific and Industrial Research (CSIR),
Government of India and the J. C. Bose fellowship to RVS from
the Department of Science and Technology (DST), Government
of India. SD acknowledges the Council of Scientific and
Industrial Research (CSIR), Government of India for a Ph.D. fellowship. We thank T. Lavanya and S. Pillai for critical reviewing
of the manuscript.
 
Date 2019-05-21T09:52:55Z
2019-05-21T09:52:55Z
2019
 
Type Article
 
Identifier Molecular Plant Pathology, 20(7): 976-989
1364-3703
http://223.31.159.10:8080/jspui/handle/123456789/952
https://onlinelibrary.wiley.com/doi/full/10.1111/mpp.12807
https://doi.org/10.1111/mpp.12807
 
Language en_US
 
Format application/pdf
 
Publisher John Wiley & Sons