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Current approaches to measure nitric oxide in plants

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Title Current approaches to measure nitric oxide in plants
 
Creator Vishwakarma, Abhaypratap
Wany, Aakanksha
Pandey, Sonika
Bulle, Mallesham
Kumari, Aprajita
Kishorekumar, Reddy
Igamberdiev, Abir U.
Mur, Luis A.J.
Gupta, Kapuganti Jagadis
 
Subject Nitric oxide
Quantum cascade laser
chemiluminiscence
hemoglobin
mitochondria
redox
 
Description Accepted date: 18 May 2019
Nitric oxide (NO), is now established as an important signalling molecule in plants where they influence growth, development and responses to stress. Despite of extensive research, the most appropriate methods to measure and localise these signalling radicals are debated and still needs investigation. Many confounding factors such as presence of other reactive intermediates, scavenging enzymes and compartmentation, influence how accurately each can be measured. Further, these signalling radicals have short half-lives ranging from seconds to minutes based on the cellular redox condition. Hence, it is necessary to use the sensitive and specific methods in order to understand the contribution of each signalling molecule to various biological processes. In this review, we will provide current state knowledge on NO measurement in plant samples, via various methods. We will also discuss advantages, limitations and wider applications of each method.
This work is funded by Ramalingaswami Fellowship and IYBA, SERB ECR to KJG.
 
Date 2019-05-22T11:05:34Z
2019-05-22T11:05:34Z
2019
 
Type Article
 
Identifier Journal of Experimental Botany, 70(17): 4333-4343
1460-2431
http://223.31.159.10:8080/jspui/handle/123456789/953
https://academic.oup.com/jxb/advance-article/doi/10.1093/jxb/erz242/5491845
https://doi.org/10.1093/jxb/erz242
 
Language en_US
 
Format application/pdf
 
Publisher Oxford University Press