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Ectopic over-expression of ABA-responsive Chickpea galactinol synthase (CaGolS) gene results in improved tolerance to dehydration stress by modulating ROS scavenging

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Title Ectopic over-expression of ABA-responsive Chickpea galactinol synthase (CaGolS) gene results in improved tolerance to dehydration stress by modulating ROS scavenging
 
Creator Salvi, Prafull
Kamble, Nitin Uttam
Majee, Manoj
 
Subject Galactinol synthase
Dehydration stress tolerance
Promoter analysis
Abscisic acid responsive
Raffinose family oligosaccharides
ROS scavenging
Chickpea
 
Description Accepted date: 25 November 2019
Galactinol synthase (EC: 2.4.1.123) is a crucial enzyme of raffinose family oligosaccharides (RFO’s) biosynthesis,
essentially catalyzes the first crucial step in the raffinose biosynthetic pathway. Galactinol and raffinose accumulation along with its higher homologs such as stachyose and verbascose has been considered to participate in
plant abiotic stress tolerance. Previously, we reported the function of chickpea galactinol synthase (CaGolS)
genes in seed vigor and heat and oxidative stress tolerance. Here, we demonstrate the role and regulation of
CaGolS in response to dehydration stress in chickpea. Through transcript accumulation and promoter-gus analyses, we demonstrate that the expression of CaGolS1 and CaGolS2 are positively influenced by dehydration
stress and ABA treatment. Further, we have shown that the level of galactinol and raffinose are remarkably
enhanced in response to dehydration stress and ABA. Utilizing CaGolS1 and CaGolS2 over-expression
Arabidopsis lines, we demonstrate the role of CaGolS in dehydration stress tolerance. The biochemical and
physiological analysis revealed that CaGolS over-expressing transgenic lines exhibited improved phenotype with
respect to higher number of siliques, plant height, and rosette diameter under dehydration stress. The improved
dehydration stress tolerance was corelated with higher chlorophyll retention and relative water content of
transgenic lines. Further, lower H2O2, MDA content, and ion-leakage in transgenic lines suggest that CaGolS
mediates dehydration stress tolerance by protecting the membrane damage from ROS attack. Collectively, our
data highlight the prospect of CaGolS genes in improving dehydration stress tolerance in plants.
This work was supported by the NGCP (grant BT/AGR/CG-Phase-II/
01/2014) and the core grant of National Institute of Plant Genome Research, Department of Biotechnology (DBT), and Government of
India. We thank technicians at the NIPGR central instrumentation facility and DBT- e-Library Consortium (DeLCON) for providing required
facilities.
 
Date 2019-12-17T09:42:32Z
2019-12-17T09:42:32Z
2020
 
Type Article
 
Identifier Environmental and Experimental Botany, 171: 103957
0098-8472
http://223.31.159.10:8080/jspui/handle/123456789/1024
https://www.sciencedirect.com/science/article/pii/S0098847219315540
https://doi.org/10.1016/j.envexpbot.2019.103957
 
Language en_US
 
Format application/pdf
 
Publisher Elsevier B.V.