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Comparative nuclear proteomics analysis provides insight into the mechanism of signaling and immune response to blast disease caused by Magnoporthe oryzae in rice

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Title Comparative nuclear proteomics analysis provides insight into the mechanism of signaling and immune response to blast disease caused by Magnoporthe oryzae in rice
 
Creator Narula, Kanika
Choudhary, Pooja
Ghosh, Sudip
Elagamey, Eman
Chakraborty, Niranjan
Chakraborty, Subhra
 
Subject Nucleus
immunity
2-DE coupled mass spectrometry
comparative proteomics
protein network
rice
Magnaporthe oryzae
 
Description Accepted date: 21 November 2018
Modulation of plant immune system by extrinsic/intrinsic factors and host-specific determinants fine-tunes cellular components involving multiple organelles, particularly nucleus to mount resistance against pathogen attack. Rice blast, caused by hemibiotrophic fungus Magnaporthe oryzae, is one of the most devastating diseases that adversely affect rice productivity. However, the role of nuclear proteins and their regulation in response to M. oryzae remains unknown. Here, we elucidate the nucleus-associated immune pathways in blast resistant rice genotype. Temporal analysis of nuclear proteome was carried out using 2-DE coupled MS/MS analysis. A total of 140 immune responsive proteins (IRPs) were identified associated with nuclear reorganization, cell division, energy production/deprivation, signaling and gene regulation. We interrogated the proteome data using correlation network analysis that identified significant functional modules pointing towards immune related coinciding processes through a common mechanism of remodelling and homeostasis. Novel clues regarding blast resistance include nucleus associated redox homeostasis and glycolytic enzyme mediated chromatin organization which manipulates cell division and immunity. Taken together, our study provides evidence that coordination of nuclear function and reprogramming of host translational machinery regulate resistance mechanism against blast disease.
This work was supported by grants from National Institute of Plant
Genome Research, New Delhi, India and Department of Biotechnology (DBT), Govt. of
India (No. BT/PR10796/BRB/10/621/2008 and BT/HRD/35/01/05/2013) to S.C. K.N. was
recipient of pre-doctoral fellowship from the Council of Scientific and Industrial research
(CSIR), Govt. of India and currently is the recipient of post-doctoral fellowship from
Department of Biotechnology (DBT), Govt. of India. P.C. is the recipient of pre-doctoral
fellowship from the University Grant Commission (UGC), Govt. of India. S.G. is the
recipient of post-doctoral fellowship from Science and Engineering Research Board (SERB),
Department of Science and Technology (DST), Govt. of India. E.E. is the recipient of postdoctoral
fellowship from DBT-TWAS. Authors also thank Jasbeer Singh for illustrations and
graphical representations in the manuscript.
 
Date 2018-11-30T05:42:14Z
2018-11-30T05:42:14Z
2019
 
Type Article
 
Identifier Proteomics, 19(3): e1800188
1615-9861
http://223.31.159.10:8080/jspui/handle/123456789/900
https://onlinelibrary.wiley.com/doi/abs/10.1002/pmic.201800188
https://doi.org/10.1002/pmic.201800188
 
Language en_US
 
Format application/pdf
 
Publisher John Wiley & Sons