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Overexpression of a cell wall damage induced transcription factor, OsWRKY42, leads to enhanced callose deposition and tolerance to salt stress but does not enhance tolerance to bacterial infection

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Title Overexpression of a cell wall damage induced transcription factor, OsWRKY42, leads to enhanced callose deposition and tolerance to salt stress but does not enhance tolerance to bacterial infection
 
Creator Pillai, Shakuntala E.
Kumar, Chandan
Patel, Hitendra K.
Sonti, Ramesh V.
 
Subject Cell wall degrading enzymes
WRKY transcription factor
OsWRKY42
Callose deposition
Salinity stress
Jasmonic acid
 
Description Accepted date: 23 August 2018
Background: Members of the WRKY gene family play important roles in regulating plant responses to abiotic and
biotic stresses. Treatment with either one of the two different cell wall degrading enzymes (CWDEs), LipaseA and
CellulaseA, induces immune responses and enhances the expression of OsWRKY42 in rice. However, the role
of OsWRKY42 in CWDE induced immune responses is not known.
Results: Expression of the rice transcription factor OsWRKY42 is induced upon treatment of rice leaves with CWDEs,
wounding and salt. Overexpression of OsWRKY42 leads to enhanced callose deposition in rice and Arabidopsis but this
does not enhance tolerance to bacterial infection. Upon treatment with NaCl, Arabidopsis transgenic plants expressing
OsWRKY42 exhibited high levels of anthocyanin and displayed enhanced tolerance to salt stress. Treatment with either
cellulase or salt induced the expression of several genes involved in JA biosynthesis and response in Arabidopsis.
Ectopic expression of OsWRKY42 results in reduced expression of cell wall damage and salt stress induced
jasmonic acid biosynthesis and response genes. OsWRKY42 expressing Arabidopsis lines exhibited enhanced
tolerance to methyl jasmonate mediated growth inhibition.
Conclusion: The results presented here suggest that OsWRKY42 regulates plant responses to either cell wall
damage or salinity stress by acting as a negative regulator of jasmonic acid mediated responses.
This work was supported by grants to RVS from the Plant-Microbe and Soil
Interaction (PMSI) project of the Council of Scientific and Industrial Research
(CSIR), Government of India. SEP acknowledges the University Grants
Commission (UGC), New Delhi for PhD fellowship. This work is also
supported by a J. C. Bose fellowship to RVS from the Department of
Science and Technology, Government of India.
 
Date 2018-09-06T17:32:37Z
2018-09-06T17:32:37Z
2018
 
Type Article
 
Identifier BMC Plant Biology, 18(1): 177
1471-2229
http://223.31.159.10:8080/jspui/handle/123456789/883
https://bmcplantbiol.biomedcentral.com/articles/10.1186/s12870-018-1391-5
https://doi.org/10.1186/s12870-018-1391-5
 
Language en_US
 
Format application/pdf
 
Publisher BioMed Central Ltd