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An iron-activated citrate transporter, MtMATE67, is required for symbiotic nitrogen fixation

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Title An iron-activated citrate transporter, MtMATE67, is required for symbiotic nitrogen fixation
 
Creator Kryvoruchko, Igor S.
Routray, Pratyush
Sinharoy, Senjuti
Torres-Jerez, Ivone
Tejada-Jiménez, Manuel
Finney, Lydia A.
Nakashima, Jin
Pislariu, Catalina I.
Benedito, Vagner A.
González-Guerrero, Manuel
Roberts, Daniel M.
Udvardi, Michael K.
 
Subject Iron-Activated Citrate Transporter
Symbiotic Nitrogen Fixation
MtMATE67
 
Description Accepted date: December 21, 2017
Iron (Fe) is an essential micronutrient for symbiotic nitrogen fixation in legume nodules, where it is required for the activity of bacterial nitrogenase, plant leghemoglobin, respiratory oxidases, and other Fe proteins in both organisms. Fe solubility and transport within and between plant tissues is facilitated by organic chelators, such as nicotianamine and citrate. We have characterized a nodule-specific citrate transporter of the multidrug and toxic compound extrusion family, MtMATE67 of Medicago truncatula. The MtMATE67 gene was induced early during nodule development and expressed primarily in the invasion zone of mature nodules. The MtMATE67 protein was localized to the plasma membrane of nodule cells and also the symbiosome membrane surrounding bacteroids in infected cells. In oocytes, MtMATE67 transported citrate out of cells in an Fe-activated manner. Loss of MtMATE67 gene function resulted in accumulation of Fe in the apoplasm of nodule cells and a substantial decrease in symbiotic nitrogen fixation and plant growth. Taken together, the results point to a primary role of MtMATE67 in citrate efflux from nodule cells in response to an Fe signal. This efflux is necessary to ensure Fe(III) solubility and mobility in the apoplasm and uptake into nodule cells. Likewise, MtMATE67-mediated citrate transport into the symbiosome space would increase the solubility and availability of Fe(III) for rhizobial bacteroids.
We thank Xiaofei Cheng and JiangQi Wen for their assistance with isolation of Tnt1 mutants; Shulan Zhang for help with experiments; Frank Coker, Colleen Elles, Janie Gallaway, and Vicki Barrett for greenhouse support with M. truncatula Tnt1 lines; and Mark Taylor for backcrossing the mutants. Pascal Ratet, Kirankumar S. Mysore, and Million Tadege are acknowledged for construction of the Tnt1 mutant resource. We also thank Lina Yang for help with preparation of a high-resolution image for Figure 1. We appreciate the support from Jeremy Murray and Christopher Town with acquisition of genomic sequences. We also thank Jeremy Murray, Maria Harrison, Rebecca Dickstein, and Carroll Vance for data exchange and critical feedback.
 
Date 2018-04-03T07:18:38Z
2018-04-03T07:18:38Z
2018
 
Type Article
 
Identifier Plant Physiology, 176(3): 2315-2329
1532-2548
http://223.31.159.10:8080/jspui/handle/123456789/845
http://www.plantphysiol.org/content/176/3/2315
https://doi.org/10.1104/pp.17.01538
 
Language en_US
 
Format application/pdf
 
Publisher American Society of Plant Biologists