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Identification of miRNA-mediated drought responsive multi-tiered regulatory network in drought tolerant rice, Nagina 22

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Title Identification of miRNA-mediated drought responsive multi-tiered regulatory network in drought tolerant rice, Nagina 22
 
Creator Balyan, Sonia
Kumar, Mukesh
Mutum, Roseeta Devi
Raghuvanshi, Utkarsh
Agarwal, Priyanka
Mathur, Saloni
Raghuvanshi, Saurabh
 
Subject Drought
Plant molecular biology
Nagina 22
drought tolerant
rice
miRNA-mediated
 
Description Accepted date: 23 October 2017
Comparative characterization of microRNA-mediated stress regulatory networks in contrasting rice cultivars is critical to decipher plant stress response. Consequently, a multi-level comparative analysis, using sRNA sequencing, degradome analysis, enzymatic and metabolite assays and metal ion analysis, in drought tolerant and sensitive rice cultivars was conducted. The study identified a group of miRNAs "Cultivar-specific drought responsive" (CSDR)-miRNAs (osa-miR159f, osa-miR1871, osa-miR398b, osa-miR408-3p, osa-miR2878-5p, osa-miR528-5p and osa-miR397a) that were up-regulated in the flag-leaves of tolerant cultivar, Nagina 22 (N22) and Vandana, but down-regulated in the sensitive cultivar, Pusa Basmati 1 (PB1) and IR64, during drought. Interestingly, CSDR-miRNAs target several copper-protein coding transcripts like plantacyanins, laccases and Copper/Zinc superoxide dismutases (Cu/Zn SODs) and are themselves found to be similarly induced under simulated copper-starvation in both N22 and PB1. Transcription factor OsSPL9, implicated in Cu-homeostasis also interacted with osa-miR408-3p and osa-miR528-5p promoters. Further, N22 flag leaves showed lower SOD activity, accumulated ROS and had a higher stomata closure. Interestingly, compared to PB1, internal Cu levels significantly decreased in the N22 flag-leaves, during drought. Thus, the study identifies the unique drought mediated dynamism and interplay of Cu and ROS homeostasis, in the flag leaves of drought tolerant rice, wherein CSDR-miRNAs play a pivotal role.
Tis work was funded by the Department of Biotechnology (DBT), Government of India (Grant no. BT/PR10071/
AGR/36/31/2007 and BT/PR628/AGR/36/674/2011) and the ‘Scheme to strengthen R&D Doctoral Research
program’ of Univ. of Delhi, Delhi, India. S.C.B. sincerely thanks the University of Delhi for the UTA fellowship
for doctoral research. R.D.M. acknowledges University Grant Commission and U.R acknowledge Department of Biotechnology (DBT) for fellowships.
 
Date 2017-11-16T10:14:35Z
2017-11-16T10:14:35Z
2017
 
Type Article
 
Identifier Scientific Reports, 7(1): 15446
2045-2322
http://223.31.159.10:8080/jspui/handle/123456789/798
https://www.nature.com/articles/s41598-017-15450-1
10.1038/s41598-017-15450-1
 
Language en_US
 
Format application/pdf
 
Publisher Nature Publishing Group