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Identification, characterization, expression profiling, and virus-induced gene silencing of armadillo repeat-containing proteins in tomato suggest their involvement in tomato leaf curl New Delhi virus resistance

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Title Identification, characterization, expression profiling, and virus-induced gene silencing of armadillo repeat-containing proteins in tomato suggest their involvement in tomato leaf curl New Delhi virus resistance
 
Creator Mandal, Arunava
Mishra, Awdhesh Kumar
Dulani, Priya
Muthamilarasan, Mehanathan
Shweta, Shweta
Prasad, Manoj
 
Subject Tomato (Solanum lycopersicum)
Tomato leaf curl New Delhi virus (ToLCNDV)
Armadillo repeat family
U-box domain
Virus-induced gene silencing
 
Description Accepted date: 7 December 2017
Armadillo repeat family is well-characterized in several plant species for their involvement in multiple regulatory processes including growth, development, and stress response. We have previously shown a three-fold higher expression of ARM protein-encoding in tomato cultivar tolerant to tomato leaf curl New Delhi virus (ToLCNDV) compared to susceptible cultivar upon virus infection. This suggests the putative involvement of ARM proteins in defense response against virus infection; however, no comprehensive investigation has been performed to address this inference. In the present study, we have identified a total of 46 ARM-repeat proteins (SlARMs), and 41 U-box-containing proteins (SlPUBs) in tomato. These proteins and their corresponding genes were studied for their physicochemical properties, gene structure, domain architecture, chromosomal localization, phylogeny, and cis-regulatory elements in the upstream promoter region. Expression profiling of candidate genes in response to ToLCNDV infection in contrasting tomato cultivars showed significant upregulation of SlARM18 in the tolerant cultivar. Virus-induced gene silencing of SlARM18 in the tolerant tomato cultivar conferred susceptibility, which suggests the involvement of this gene in resistance mechanism. Further studies are underway to functionally characterize SlARM18 to delineate its precise role in defense mechanism.
The authors’ research in this area is supported by the Department of Biotechnology, Government of India [File No. BT/PR8357/PBD/16/1033/2013]. Mandal A and Shweta S acknowledge the National Post-Doctoral Fellowship received from Science and Engineering Research Board (SERB), Department of Science and Technology (DST), Govt. of India [File No. PDF/2016/000382 and PDF/2016/001430, respectively].
 
Date 2017-12-21T11:03:11Z
2017-12-21T11:03:11Z
2018
 
Type Article
 
Identifier Functional & Integrative Genomics, 18(2):101-111
1438-7948
http://223.31.159.10:8080/jspui/handle/123456789/810
https://link.springer.com/article/10.1007%2Fs10142-017-0578-4
https://doi.org/10.1007/s10142-017-0578-4
 
Language en_US
 
Format application/pdf
 
Publisher Springer Nature