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Transcriptional signatures modulating shoot apical meristem morphometric and plant architectural traits enhance yield and productivity in chickpea

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Title Transcriptional signatures modulating shoot apical meristem morphometric and plant architectural traits enhance yield and productivity in chickpea
 
Creator Narnoliya, Laxmi
Basu, Udita
Bajaj, Deepak
Malik, Naveen
Thakro, Virevol
Daware, Anurag
Sharma, Akash
Tripathi, Shailesh
Hegde, V.S.
Upadhyaya, Hari D.
Singh, Ashok K.
Tyagi, Akhilesh K.
Parida, Swarup K.
 
Subject chickpea
desi
fine mapping
GWAS
kabuli
map -based cloning
NIL
plant height
plant width
productivity
QTL
RIL
SAM
SNPs
transcription factor
yield
 
Description Accepted date: 13 February 2019
Plant height (PH) and plant width (PW), two of the major plant architectural traits determining the yield and productivity of a crop, are defined by diverse morphometric characteristics of the shoot apical meristem (SAM). The identification of potential molecular tags from a single gene that simultaneously modulates these plant/SAM architectural traits is thus prerequisite to achieve enhanced yield and productivity in crop plants, including chickpea. Large‐scale multi‐environment phenotyping of the association panel and mapping population have ascertained the efficacy of three vital SAM morphometric trait parameters, SAM width, SAM height and SAM area, as key indicators to unravel the genetic basis of the wide PW and PH trait variations observed in desi chickpea. The present study integrated a genome‐wide association study (GWAS); QTL/fine‐mapping and map‐based cloning with molecular haplotyping; transcript profiling; and protein‐DNA interaction assays for the dissection of plant architectural traits in chickpea. These exertions delineated natural alleles and superior haplotypes from a CabHLH121 transcription factor (TF) gene within the major QTLs governing PW, PH and SAM morphometric traits. A genome‐wide protein‐DNA interaction assay assured the direct binding of a known stem cell master regulator, CaWUS, to the WOX‐homeodomain TF binding sites of a CabHLH121 gene and its constituted haplotypes. The differential expression of CaWUS and transcriptional regulation of its target CabHLH121 gene/haplotypes were apparent, suggesting their collective role in altering SAM morphometric characteristics and plant architectural traits in the contrasting near isogenic lines (NILs). The NILs introgressed with a superior haplotype of a CabHLH121 exhibited optimal PW and desirable PH as well as enhanced yield and productivity without compromising any component of agronomic performance. These molecular signatures of the CabHLH121 TF gene have the potential to regulate both PW and PH traits through the modulation of proliferation, differentiation and maintenance of the meristematic stem cell population in the SAM; therefore, these signatures will be useful in the translational genomic study of chickpea genetic enhancement. The restructured cultivars with desirable PH (semi‐dwarf) and PW will ensure maximal planting density in a specified cultivable field area, thereby enhancing the overall yield and productivity of chickpea. This can essentially facilitate the achievement of better remunerative outputs by farmers with rational land use, thus ensuring global food security in the present scenario of an increasing population density and shrinking per capita land area.
We are very much grateful to Mr. Sube Singh, lead scientific officer, Grain Legumes
Research Program/Genebank, ICRISAT, Hyderabad for assisting in collecting multi
-environments field phenotyping data of germplasm accessions and mapping population.
Constant assistance provided by all the scientific and technical staffs of NIPGR and IARI,
New Delhi and ICRISAT, Hyderabad to conduct these research works is acknowledged. We
are also thankful to central instrumentation facility (CIF), plant growth facility (PGF) and
DBT-eLibrary Consortium (DeLCON) of NIPGR, New Delhi for providing timely support
and access to e-resources for this research work. The financial support for this study is provided by a research grant from the Department of
Biotechnology (DBT), Government of India. LN, UB, VT and AD acknowledge the UGC
(University Grants Commission) and DBT, India for research fellowship awards.
 
Date 2019-02-18T07:26:16Z
2019-02-18T07:26:16Z
2019
 
Type Article
 
Identifier Plant Journal, 98(5): 864-883
1365-313X
http://223.31.159.10:8080/jspui/handle/123456789/919
https://onlinelibrary.wiley.com/doi/abs/10.1111/tpj.14284
https://doi.org/10.1111/tpj.14284
 
Language en_US
 
Format application/pdf
 
Publisher John Wiley & Sons