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Using foldscope to monitor superoxide production and cell death during pathogen infection in arabidopsis under different nitrogen regimes

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Title Using foldscope to monitor superoxide production and cell death during pathogen infection in arabidopsis under different nitrogen regimes
 
Creator Arora, Reena
Singh, Pooja
Kumari, Aprajita
Pathak, Pradeep Kumar
Gupta, Kapuganti Jagadis
 
Subject Foldscope
Nitrate
Ammonium
Arabidopsis
Pseudomonas syringae
Fusarium oxysporum
 
Description Accepted date: 09 October 2019
Nitrogen nutrition plays a role in plant growth development and resistance against biotic and abiotic stress. During pathogen infection various signal molecules such as reactive oxygen species, calcium, reactive nitrogen species, salicylic acid, and ethylene plays an important role. The form of nitrogen nutrition such as nitrate or ammonium plays a role in production of these molecules. Under nitrate nutrition NO is predominant. The produced NO plays a role in reacting with superoxide to generate peroxynitrite to induce cell death during hypersensitive response elicited by avirulent pathogens. Excess of ROS is also detrimental to plants and NO plays a role in regulating ROS. Hence it is important to observe superoxide production during infection. By using an avirulent Pseudomonas syringae and Arabidopsis differential N nutrition we show superoxide production in leaves using a paper microscope called Foldscope, which can be applied as a simple microscope to observe objects. The data also compared with root system infected with pathogenic Fusarium oxysporum. Taken together here we show that Foldscope is a cost-effective and powerful technique to visualize superoxide and cell death in plants during infection.
This work is supported by Foldscope project funded by DBT-Prakash lab initiative. PS and PKP are funded by UGC SRF and SRF.
 
Date 2019-10-15T05:10:38Z
2019-10-15T05:10:38Z
2020
 
Type Book chapter
 
Identifier Methods in Molecular Biology, 2057: 93-102
978-1-4939-9790-9
http://223.31.159.10:8080/jspui/handle/123456789/996
https://link.springer.com/protocol/10.1007%2F978-1-4939-9790-9_9
https://doi.org/10.1007/978-1-4939-9790-9_9
 
Language en_US
 
Format application/pdf
 
Publisher Springer Nature Publishing AG