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Transcriptional regulation of chickpea ferritin CaFer1 influences its role in iron homeostasis and stress response

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Title Transcriptional regulation of chickpea ferritin CaFer1 influences its role in iron homeostasis and stress response
 
Creator Parveen, Shaista
Pandey, Aarti
Jameel, Neha
Chakraborty, Niranjan
Chakraborty, Subhra
 
Subject cis- and trans-acting elements
Iron-responsive genes
Phylogenetic relationship
Phytoferritin
Protein-protein interaction
Transcriptional regulation
 
Description Accepted date: 22 December 2017
Ferritin, ubiquitous among all living organisms except yeast, exhibits iron-regulated expression. In plants, this
regulation is applied through transcriptional control. Previous studies established the presence of two types of
cis-acting elements in the promoter region: the iron regulatory element (FRE) in soybean and the iron-dependent
regulatory sequence (IDRS) in maize and Arabidopsis. Adverse environmental conditions (e.g. water-deficit and
oxidative stress) are known to modulate the expression of phytoferritin genes. In this study, we cloned and
investigated the promoter sequence of a chickpea ferritin, designated CaFer1. Phylogenetic analysis of the CaFer1
promoter revealed its evolutionary relationship with other phytoferritins. The CaFer1 promoter exhibited several
putative regulatory elements including two known transcription factor (TF) binding sites, Athb-1 and Myb.Ph.
Electrophoretic mobility shift assay confirmed the sequence-specific binding of Athb-1 and Myb.Ph on the
CaFer1 promoter. The TF-binding dynamics of CaFer1 showed high induction under conditions of iron-deficiency
and water-deficit. We also demonstrated the possible interaction of CaFer1 with IRT1, a key component of the
iron uptake system in plants, indicating its involvement in maintaining cellular iron levels. These results provide
new insights into the underlying mechanisms of function of these interacting factors in CaFer1-mediated iron
homeostasis and the stress response in plants.
This work was supported by a grant (BT/AGR/CG-Phase-II/01/2014) from the Department of Biotechnology (DBT), Govt. of India. The authors thank the DBT, Govt. of India for providing pre-doctoral fellowship to S.P. A.P. received postdoctoral fellowship from the National Institute of Plant Genome Research, New Delhi.
 
Date 2018-01-08T10:37:52Z
2018-01-08T10:37:52Z
2018
 
Type Article
 
Identifier Journal of Plant Physiology, 222: 9-16
0176-1617
http://223.31.159.10:8080/jspui/handle/123456789/822
https://www.sciencedirect.com/science/article/pii/S0176161717303115
https://doi.org/10.1016/j.jplph.2017.12.015
 
Language en_US
 
Format application/pdf
 
Publisher Elsevier B.V.