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CBL-interacting protein kinase 25 contributes to root meristem development

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Title CBL-interacting protein kinase 25 contributes to root meristem development
 
Creator Meena, Mukesh Kumar
Vishwakarma, Niraj Kumar
Tripathi, Vineeta
Chattopadhyay, Debasis
 
Subject auxin
CIPK25
cytokinin
meristem
PIN
root
SHY2
 
Description Accepted date: 18 Sept 2018
Coordination of auxin and cytokinin activities determines root meristem size during post-embryonic development. Calcineurin B-like proteins (CBLs) and their interacting protein kinases (CIPKs) constitute signaling modules that relay calcium signals. Here we report that CIPK25 is involved in regulating the root meristem size. Arabidopsis plants lacking CIPK25 expression displayed a short root phenotype and a slower root growth rate with less meristem cells. This phenotype was rescued by restoration of CIPK25 expression. CIPK25 interacted with CBL4 and -5, and displayed a strong gene expression in the flower and root except in the cell proliferation domain in the root apical meristem. Its expression in root was positively and negatively regulated by auxin and cytokinin, respectively. cipk25 T-DNA insertion line was compromised in auxin transport and auxin-responsive promoter activity. cipk25 mutant line showed altered expression of auxin efflux carriers and an Aux/IAA family gene SHY2. Decreased PIN1 and PIN2 expression in cipk25 mutant line was completely restored when combined with SHY2 loss-of-function mutation resulting in recovery of root growth. SHY2 and PIN1 expression was partially regulated by cytokinin even in absence of CIPK25, suggesting existence of CIPK25-independent cytokinin signaling pathway(s) as well. Our results suggested a role of CIPK25 in root meristem development.
This project was funded by National Institute of Plant Genome Research (NIPGR), an
autonomous institute funded by the Department of Biotechnology (DBT), Government of
India and by DBT under the Next Generation Challenge Programme on Chickpea Genomics
(grant no. BT/PR12919/AGR/02/676/2009 from 2009-14). Authors acknowledge Dr. J. W.
Reed and Dr. Punita Nagpal of Department of Biology, University of North Carolina, Chapel
Hill, USA (UNC) for their scientific and technical suggestions and the plant lines and Dr. J.
Vadassery, NIPGR for critical reading of the manuscript. MKM, NKV and VT acknowledge
Council for Scientific and Industrial Research (CSIR), India and DBT, respectively, for
fellowships. The authors are thankful to DBT-eLibrary Consortium (DeLCON) for providing access to e-Resources. Authors also acknowledge central instrument facility at NIPGR for
instrumentation support.
 
Date 2018-09-25T08:04:58Z
2018-09-25T08:04:58Z
2019
 
Type Article
 
Identifier Journal of Experimental Botany, 70(1): 133-147
1460-2431
http://223.31.159.10:8080/jspui/handle/123456789/887
https://academic.oup.com/jxb/advance-article/doi/10.1093/jxb/ery334/5099575
https://doi.org/10.1093/jxb/ery334
 
Language en_US
 
Format application/pdf
 
Publisher Oxford University Press