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A strong early acting wound-inducible promoter, RbPCD1pro, activates cryIAc expression within minutes of wounding to impart efficient protection against insects

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Title A strong early acting wound-inducible promoter, RbPCD1pro, activates cryIAc expression within minutes of wounding to impart efficient protection against insects
 
Creator Pandey, Saurabh Prakash
Singh, Amar Pal
Srivastava, Shruti
Chandrashekar, Krishnappa
Sane, Aniruddha P.
 
Subject insect resistance
constitutive promoter
GUS
jasmonic acid
Bacillus thuringiensis
tomato
 
Description Accepted date: 12 December 2018
The expression of insecticidal proteins under constitutive promoters in transgenic plants is
fraught with problems like developmental abnormalities, yield drag, expression in unwanted
tissues, and seasonal changes in expression. RbPCD1pro, a rapid, early acting wound-inducible
promoter from rose that is activated within 5 min of wounding, was isolated and characterized.
Wounding increased transcript levels up to 150 and 500 folds within 5 and 20 min coupled with
high translation as seen by histochemical GUS enzyme activity within 5–20 min. RbPCD1pro was
activated by both sucking and chewing insects and showed wound-inducible expression in
various aerial tissues of plants representing commercially important dicot and monocot families.
The promoter showed no expression in any vegetative tissue except upon wounding.
Functionality of RbPCD1pro was tested by its ability to drive expression of the insecticidal protein
gene cryIAc in transgenic Arabidopsis and tomato. Strong wound-inducible CryIAc expression
was observed in both plants that increased 100–350 fold (Arabidopsis) and 280–600 fold
(tomato) over the unwounded background within 5 min and over 1000–1600 fold within
20 min. The unwounded background level was just 3–6% of the CaMV35S promoter while
wound-induced expression was 5–27 folds higher than the best CaMV35S line in just 5 min and
80-fold higher in 20 min. Transgenic plants showed strong resistance even to larger fourth instar
larvae of H. armigera and no abnormalities in development and general plant growth. This is one
of the earliest acting promoters with wide biotechnological application across monocot and dicot
plants.
We thank Dr PK Singh (Dept of Genetics and Plant Molecular
Biology, CSIR-NBRI) for the gift of the cryIAc gene and protein
and Dr Priya Singh for help with the studies. We also thank Mr
SMH Abidi and Mr Rakesh Srivastava for rearing the insects and
help with insect bioassays and Mr Ram Awadh for care of the
transgenic tomato plants. The Council of Scientific and Industrial
Research (CSIR), New Delhi, Govt of India funded the work under
the projects NWP-03 and BSC0107 and supported SPP and APS
with Senior Research Fellowships.
 
Date 2019-07-01T10:58:04Z
2019-07-01T10:58:04Z
2019
 
Type Book
 
Identifier Plant Biotechnology Journal, 17(7): 1458-1470
1467-7652
http://223.31.159.10:8080/jspui/handle/123456789/960
https://onlinelibrary.wiley.com/doi/full/10.1111/pbi.13071
https://doi.org/10.1111/pbi.13071
 
Language en_US
 
Format application/pdf
 
Publisher John Wiley & Sons