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Chilli leaf curl virus infection downregulates the expression of the genes encoding chloroplast proteins and stress-related proteins

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Title Chilli leaf curl virus infection downregulates the expression of the genes encoding chloroplast proteins and stress-related proteins
 
Creator Kushwaha, Nirbhay Kumar
Mansi
Sahu, Pranav Pankaj
Prasad, Manoj
Chakrabroty, Supriya
 
Subject Geminivirus
Chilli leaf curl virus
Suppression subtractive hybridization
Capsicum annuum
Chloroplast
 
Description Accepted date: 16 July 2019
Virus infection alters the expression of several
host genes involved in various cellular and biological
processes in plants. Most of the studies performed till now
have mainly focused on genes which are up-regulated and
later projected them as probable stress tolerant/susceptible
genes. Nevertheless, genes which are down-regulated
during plant-virus interaction could also play a critical role
on disease development as well as in combating the virus
infection. Hence, to identify such down-regulated genes
and pathway, we performed reverse suppression subtractive hybridization in Capsicum annuum var. Punjab Lal
following Chilli leaf curl virus (ChiLCV) infection. The
screening and further processing suggested that majority of
the genes (approximately 35% ESTs) showed homology
with the genes encoding chloroplast proteins and 16%
genes involved in the biotic and abiotic stress response.
Additionally, we identified several genes, functionally
known to be involved in metabolic processes, protein
synthesis and degradation, ribosomal proteins, energy
production, DNA replication and transcription, and transporters. We also found 3% transcripts which did not show
homology with any known genes. The redundancy analysis
revealed the maximum percentage of chlorophyll a-b
binding protein (15/96) and auxin-binding proteins (13/96).
Funding was provided by University Grants Commission
[Grant Nos. UGC-RNW (SLS/SC/2016, UGC-SAP (SLS/SAP/SC/
2016)], Ministry of Science and Technology [Grant No. DST-FIST
(JNU/SLS/SC/FIST-16)].
 
Date 2019-10-03T11:02:21Z
2019-10-03T11:02:21Z
2019
 
Type Article
 
Identifier Physiology and Molecular Biology of Plants, 25(5): 1185-1196
0974-0430
http://223.31.159.10:8080/jspui/handle/123456789/992
https://link.springer.com/article/10.1007%2Fs12298-019-00693-1
https://doi.org/10.1007/s12298-019-00693-1
 
Language en_US
 
Format application/pdf
 
Publisher Springer Nature Publishing AG