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Low temperature enhanced flavonol synthesis requires light-associated regulatory components in Arabidopsis thaliana

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Title Low temperature enhanced flavonol synthesis requires light-associated regulatory components in Arabidopsis thaliana
 
Creator Bhatia, Chitra
Pandey, Ashutosh
Gaddam, Subhash Reddy
Hoecker, Ute
Trivedi, Prabodh Kumar
 
Subject COP1
flavonol synthesis
HY5
light regulation
low temperature stress
MYB transcription factors
 
Description Accepted date: 6 July 2018
Plants are continuously exposed to myriad of stresses, which lead to the formation of secondary metabolites including flavonoids. Studies suggest that low temperature exposure leads to enhanced flavonoid accumulation in Arabidopsis thaliana. In addition, flavonoid biosynthesis is regulated by light through various regulatory factors. Therefore, plant may possess the capability to integrate light and low temperature signals for survival under freezing conditions. However, detailed molecular mechanism and the regulatory factors associated with light- and low temperature- responsive flavonoid biosynthesis remain largely unknown. Here, we report a strict requirement of light for the low temperature- enhanced flavonol biosynthesis. Low temperature-induced expression of biosynthetic genes as well as flavonol accumulation was hampered in ELONGATED HYPOCOTYL (hy5) and myb11myb111myb12 triple mutants as compared to wild-type (WT) in Arabidopsis. Over-expression of AtHY5 in the hy5 mutant restored induction of gene expression and flavonol accumulation in response to low-temperature in light. Metabolite and gene expression analysis also suggests negative role of CONSTITUTIVE PHOTOMORPHOGENIC1 (COP1) in accumulation of flavonols in response to low temperature. Over-expression of AtMYB12 enhanced accumulation of flavonols under low-temperature in a light-dependent manner. Together, our analysis suggests requirement of HY5 and flavonol-specific MYB regulatory factors for low temperature-induced flavonol synthesis.
Research was supported by Council of Scientific and Industrial Research, New Delhi in the
form of Network Project (BSC-0107) and CSIR-BMBF collaborative project. CB, SRG
acknowledge University Grant Commission, New Delhi for Senior and Junior Research
Fellowship respectively.
 
Date 2018-07-19T11:48:28Z
2018-07-19T11:48:28Z
2018
 
Type Article
 
Identifier Plant and Cell Physiology, 59(10): 2099-2112
1471-9053
http://223.31.159.10:8080/jspui/handle/123456789/876
https://academic.oup.com/pcp/advance-article/doi/10.1093/pcp/pcy132/5053954
https://doi.org/10.1093/pcp/pcy132
 
Language en_US
 
Format application/pdf
 
Publisher Oxford University Press