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The Ca2+ channel CNGC19 regulates Arabidopsis defense against spodoptera herbivory

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Title The Ca2+ channel CNGC19 regulates Arabidopsis defense against spodoptera herbivory
 
Creator Meena, Mukesh Kumar
Prajapati, Ramgopal
Krishna, Deepthi
Divakaran, Keerthi
Pandey, Yogesh
Reichelt, Michael
Mathew, M.K.
Boland, Wilhelm
Mithöfer, Axel
Vadassery, Jyothilakshmi
 
Subject Ca2+
Spodoptera Herbivory
CNGC19
Arabidopsis
 
Description Accepted date: May 7, 2019
Cellular calcium elevation is an important signal used by plants for recognition and signaling of environmental stress. Perception of the generalist insect, Spodoptera litura, by Arabidopsis thaliana activates cytosolic Ca2+ elevation, which triggers downstream defense. However, not all the Ca2+ channels generating the signal have been identified, nor are their modes of action known. We report on a rapidly activated, leaf vasculature- and plasma membrane-localized, CYCLIC NUCLEOTIDE GATED CHANNEL19 (CNGC19), which activates herbivory-induced Ca2+ flux and plant defense. Loss of CNGC19 function results in decreased herbivory defense. The cngc19 mutant shows aberrant and attenuated intra-vascular Ca2+ fluxes. CNGC19 is a Ca2+ permeable channel, as hyperpolarization of CNGC19-expressing Xenopus oocytes in the presence of both cAMP and Ca2+ results in Ca2+ influx. Breakdown of Ca2+-based defence in cngc19 mutants leads to a decrease in herbivory-induced JA-Ile biosynthesis and expression of JA responsive genes. cngc19 mutants are deficient in aliphatic glucosinolate accumulation and hyperaccumulate its precursor, methionine. CNGC19 modulates aliphatic glucosinolate biosynthesis in tandem with BRANCHED-CHAIN AMINO ACID TRANSAMINASE4 (BCAT4), which is involved in the chain elongation pathway of Met-derived glucosinolates. Furthermore, CNGC19 interacts with herbivory-induced CALMODULIN2 (CaM2) in planta. Together, our work reveals a key mechanistic role for the Ca2+ channel CNGC19 in the recognition of herbivory and the activation of defense signaling.
This work was funded by the Department of Biotechnology (DBT), India through NIPGR core and BioCare
grants (BT/PR18059/BIC/101/994/2016), MPG-India partner group program of the Max Planck Society
(Germany) and Department of Science and Technology (DST), India (DST/INT/MPG/P-28/2015). We
acknowledge DBT-JRF for funding R.P, National Bureau of Agricultural Insect Resources (NBAIR),
Bangalore for S. litura eggs (National Accession No. is: NBAII-MP-NOC-02), Pradeep Kumar Maurya
(NIPGR) for rearing Spodoptera, NIPGR central instrumentation and phytotron facility, DBT-eLibrary
Consortium (DeLCON) for providing access to e-resources, Sahil Lall and Central Imaging and Flow
Facility (NCBS) for Xenopus oocyte images, Dr. Suneel Katariya (JNU) for help with cAMP quantification,
Monika Heyer and Andrea Lehr (MPI-CE) for help with phytohormone extraction, Prof. Dale Sanders (JIC,
UK) for GCaMP3 lines, Prof. Gerald Berkowitz (University of Connecticut, USA) for pepr1 pepr2 mutant,
Prof. Stefan Binder (University of Ulm, Germany) for bcat4 mutant line SALK_013627 and Dr. Guillermo Hugo Jimenez-Aleman (MPI-CE, Jena) for chemically synthesized JA-Ile.
 
Date 2019-05-13T09:50:18Z
2019-05-13T09:50:18Z
2019
 
Type Article
 
Identifier Plant Cell, 31(7): 1539-1562
1532-298X
http://223.31.159.10:8080/jspui/handle/123456789/948
http://www.plantcell.org/content/early/2019/05/10/tpc.19.00057
https://doi.org/10.1105/tpc.19.00057
 
Language en_US
 
Format application/pdf
 
Publisher American Society of Plant Biologists