Record Details

Characterization of DEAD-box family of RNA helicases in tomato provides insights into their roles in biotic and abiotic stresses

NIPGR Digital Knowledge Repository (NDKR)

View Archive Info
 
 
Field Value
 
Title Characterization of DEAD-box family of RNA helicases in tomato provides insights into their roles in biotic and abiotic stresses
 
Creator Pandey, Saurabh
Muthamilarasan, Mehanathan
Sharma, Namisha
Chaudhry, Vaishali
Dulani, Priya
Shweta, Shweta
Jha, Sarita
Mathur, Saloni
Prasad, Manoj
 
Subject RNA helicase
DEAD-box RNA helicase
Disease resistance
Tomato leaf curl New Delhi virus
Abiotic stress
Tomato (Solanum lycopersicum)
 
Description Accepted date: 27 November 2018
In plants, RNA helicases play significant roles in growth, development and stress response. In a previous study, a
three-fold upregulation of a DEAD-box RNA helicase in a tomato cultivar tolerant to Tomato leaf curl New Delhi
virus (ToLCNDV) as compared to susceptible cultivar during virus infection was shown. Given this, a comprehensive study was performed to identify the members of RNA helicase family in tomato and analyze their
functional properties in response to abiotic stresses, hormone treatments and ToLCNDV infection. A total of 131
genes were identified and classified into DEAD- (42), DEAH- (38), and DExD/H-box (51) RNA helicases.
Expression profiling of candidate genes in response to abiotic stresses and ToLCNDV infection in contrasting
tomato cultivars suggested the putative roles of SlDEAD23 and SlDEAD35 in biotic and abiotic stresses.
Heterologous overexpression of these genes in yeast enhanced the tolerance of transgenic cells to salt and cold
stresses. Further, virus-induced silencing of SlDEAD35 in ToLCNDV tolerant cultivar resulted in susceptibility to
virus infection, thus suggesting its involvement in tolerance mechanism. Altogether, this study provides novel
insights into the structure, organization and involvement of DEAD-box RNA helicase genes in biotic and abiotic
stress responses in tomato.
This work was supported by the Core Grant of National Institute of
Plant Genome Research, New Delhi, India. Saurabh Pandey acknowledges the Department of Biotechnology, Govt. of India for the research
fellowship. Mehanathan Muthamilarasan acknowledge the DST
INSPIRE Faculty Award from Department of Science and Technology,
Govt. of India. Authors also acknowledge Prof. Savithramma DineshKumar, Plant Biology Department, University of California, Davis, USA
for providing TRV-VIGS vectors. The authors are also thankful to DBTeLibrary Consortium (DeLCON) for providing access to the e-resources.
 
Date 2019-01-02T05:57:12Z
2019-01-02T05:57:12Z
2019
 
Type Article
 
Identifier Environmental and Experimental Botany, 158: 107-116
0098-8472
http://223.31.159.10:8080/jspui/handle/123456789/906
https://www.sciencedirect.com/science/article/pii/S009884721830950X
https://doi.org/10.1016/j.envexpbot.2018.11.018
 
Language en_US
 
Format application/pdf
 
Publisher Elsevier B.V.