Record Details

CRISPR-Cas9 system: A new-fangled dawn in gene editing

NIPGR Digital Knowledge Repository (NDKR)

View Archive Info
 
 
Field Value
 
Title CRISPR-Cas9 system: A new-fangled dawn in gene editing
 
Creator Gupta, Darshana
Bhattacharjee, Oindrila
Mandal, Drishti
Sen, Madhab Kumar
Dey, Dhritiman
Dasgupta, Adhiraj
Kazi, Tawsif Ahmed
Gupta, Rahul
Sinharoy, Senjuti
Acharya, Krishnendu
Chattopadhyay, Dhrubajyoti
Ravichandiran, V.
Roy, Syamal
Ghosh, Dipanjan
 
Subject CRISPR-Cas9
Genome editing
Knock out
Knock in
 
Description Accepted date: 5 July 2019
Till date, only three techniques namely Zinc Finger Nuclease (ZFN), Transcription-Activator Like Effector Nucleases (TALEN) and Clustered Regularly Interspaced Short Palindromic Repeats-CRISPR-Associated 9 (CRISPR-Cas9) are available for targeted genome editing. CRISPR-Cas system is very efficient, fast, easy and cheap technique for achieving knock-out gene in the cell. CRISPR-Cas9 system refurbishes the targeted genome editing approach into a more expedient and competent way, thus facilitating proficient genome editing through embattled double-strand breaks in approximately any organism and cell type. The off-target effects of CRISPR Cas system has been circumnavigated by using paired nickases. Moreover, CRISPR-Cas9 has been used effectively for numerous purposes, like knock-out of a gene, regulation of endogenous gene expression, live-cell labelling of chromosomal loci, edition of single-stranded RNA and high-throughput gene screening. The execution of the CRISPR-Cas9 system has amplified the number of accessible scientific substitutes for studying gene function, thus enabling generation of CRISPR-based disease models. Even though many mechanistic questions are left behind to be answered and the system is not yet fool-proof i.e., a number of challenges are yet to be addressed, the employment of CRISPR-Cas9–based genome engineering technologies will increase our understanding to disease processes and their treatment in the near future. In this review we have discussed the history of CRISPR-Cas9, its mechanism for genome editing and its application in animal, plant and protozoan parasites. Additionally, the pros and cons of CRISPR-Cas9 and its potential in therapeutic application have also been detailed here.
Authors acknowledge the funding agencies: DBT, Govt of India (Grant No - BT/PR 26301/GET/119/258/2017) and WB-DBT (63 (Sanc.)-BT/P/Budget/RD-74/2017). S. Roy is supported by JC Bose Fellowship SB/S2/JCB-65/2014.
 
Date 2019-09-05T11:42:57Z
2019-09-05T11:42:57Z
2019
 
Type Article
 
Identifier Life Sciences, 232: 116636
0024-3205
http://223.31.159.10:8080/jspui/handle/123456789/977
https://www.sciencedirect.com/science/article/pii/S0024320519305624?via%3Dihub
https://doi.org/10.1016/j.lfs.2019.116636
 
Language en_US
 
Format application/pdf
 
Publisher Elsevier B.V.