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Dehydration-induced alterations in chloroplast proteome and reprogramming of cellular metabolism in developing chickpea delineate interrelated adaptive responses

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Title Dehydration-induced alterations in chloroplast proteome and reprogramming of cellular metabolism in developing chickpea delineate interrelated adaptive responses
 
Creator Lande, Nilesh Vikam
Barua, Pragya
Gayen, Dipak
Kumar, Sunil
Varshney, Swati
Sengupta, Shantanu
Chakraborty, Subhra
Chakraborty, Niranjan
 
Subject Chloroplasts
Metabolic reprograming
Quantitative proteomics
Retrograde signaling
Transcript abundance
Water-deficit stress
 
Description Accepted date: 20 Nov 2019
Chloroplast, the energy organelle unique to photosynthetic eukaryotes, executes several crucial functions including photosynthesis. While chloroplast development and function are controlled by the nucleus, environmental stress modulated alterations perceived by the chloroplasts are communicated to the nucleus via retrograde signaling. Notably, coordination of chloroplast and nuclear gene expression is synchronized by
anterograde and retrograde signaling. The chloroplast proteome holds significance for stress responses and
adaptation. We unraveled dehydration-induced alterations in the chloroplast proteome of a grain legume,
chickpea and identified an array of dehydration-responsive proteins (DRPs) primarily involved in photosynthesis, carbohydrate metabolism and stress response. Notably, 12 DRPs were encoded by chloroplast genome,
while the rest were nuclear-encoded. We observed a coordinated expression of different multi-subunit protein
complexes viz., RuBisCo, photosystem II and cytochrome b6f, encoded by both chloroplast and nuclear genome.
Comparison with previously reported stress-responsive chloroplast proteomes showed unique and overlapping
components. Transcript abundance of several previously reported markers of retrograde signaling revealed relay
of dehydration-elicited signaling events between chloroplasts and nucleus. Additionally, dehydration-triggered
metabolic adjustments demonstrated alterations in carbohydrate and amino acid metabolism. This study offers a
panoramic catalogue of dehydration-responsive signatures of chloroplast proteome and associated retrograde
signaling events, and cellular metabolic reprograming.
This work was supported by grants from the Department of
Biotechnology (DBT), India (BT/AGR/CG-PhaseII/01/2014) and
National Institute of Plant Genome Research, New Delhi to N.C. We
thank Department of Science and Technology (DST), India for SERBNational Post-Doctoral Fellowship (PDF/2016/002615) granted to D.G.
and Department of Biotechnology (DBT) for providing pre-doctoral
fellowship to P.B. We acknowledge Council of Scientific and Industrial
Research (CSIR), India for providing pre-doctoral fellowship to N.V.L.
We thank Rahul Chakraborty, for raw data submission in
ProteomeXchange database. We also thank Mr. Jasbeer Singh for illustrations and graphical representation in the manuscript.
 
Date 2019-12-12T10:36:16Z
2019-12-12T10:36:16Z
2020
 
Type Article
 
Identifier Plant Physiology and Biochemistry, 146: 337-348
0981-9428
http://223.31.159.10:8080/jspui/handle/123456789/1020
https://www.sciencedirect.com/science/article/pii/S0981942819304991?via%3Dihub
https://doi.org/10.1016/j.plaphy.2019.11.034
 
Language en_US
 
Format application/pdf
 
Publisher Elsevier B.V.