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Quantitative extracellular matrix proteomics suggests cell wall reprogramming in host-specific immunity during vascular wilt caused by Fusarium oxysporum in chickpea

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Title Quantitative extracellular matrix proteomics suggests cell wall reprogramming in host-specific immunity during vascular wilt caused by Fusarium oxysporum in chickpea
 
Creator Elagamey, Eman
Narula, Kanika
Sinha, Arunima
Ghosh, Sudip
Abdellatef, Magdi A.E.
Chakraborty, Niranjan
Chakraborty, Subhra
 
Subject Chickpea
Comparative proteomics and protein network
Extracellular matrix
Fusarium oxysporum
Innate immunity
Vascular wilt
 
Description Accepted date: 2 November 2017
Extracellular matrix (ECM) is the unique organelle that perceives stress signals and reprograms molecular events of host cell during patho-stress. However, our understanding of how ECM dictates plant immunity is largely unknown. Vascular wilt caused by the soil borne filamentous fungus Fusarium oxysporum is a major impediment for global crop productivity. To elucidate the role of ECM proteins and molecular mechanism associated with cell wall mediated immunity, the temporal changes of ECM proteome was studied in vascular wilt resistant chickpea cultivar upon F. oxysporum infection. The 2-DE protein profiling coupled with mass spectrometric analysis identified 166 immune responsive proteins (IRPs) involved in variety of functions. Our data suggest that wall remodeling; protein translocation, stabilization and chitin triggered immunity; extracellular ATP signaling are major players in early, middle and later phases of ECM signaling during fungal attack. Furthermore, we interrogated the proteome data using network analysis that identified modules enriched in known and novel immunity-related prognostic proteins centered around nascent aminopolypeptide complex (NAC), amine oxidase, thioredoxin and chaperonin. This study for the first time provides an insight into the complex network operating in the ECM and impinges on the surveillance mechanism of innate immunity during patho-stress in crop plant.
This work was supported by grants from National Institute of Plant Genome Research, New Delhi, India and Department of Biotechnology, Govt. of India (No. BT/PR10796/BRB/10/621/2008 and BT/HRD/35/01/05/2013) to S.C. E.E. was the recipient of pre-doctoral fellowship from DBT-TWAS. M.A. was the recipient of post-doctoral fellowship from DBT-TWAS. K.N. is the recipient of post-doctoral fellowship from Department of Biotechnology (DBT), Govt. of India. A.S. is the recipient of pre-doctoral fellowship from the Council of Scientific and Industrial research (CSIR), Govt. of India. Authors also thank Jasbeer Singh for illustrations and graphical representations in the manuscript.
 
Date 2017-11-21T04:57:03Z
2017-11-21T04:57:03Z
2017
 
Type Article
 
Identifier Proteomics, 17(23-24): 1600374
1615-9861
http://223.31.159.10:8080/jspui/handle/123456789/801
http://onlinelibrary.wiley.com/doi/10.1002/pmic.201600374/abstract
10.1002/pmic.201600374
 
Language en_US
 
Format application/pdf
 
Publisher John Wiley & Sons