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Spodoptera litura-mediated chemical defense is differentially modulated in older and younger systemic leaves of Solanum lycopersicum

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Title Spodoptera litura-mediated chemical defense is differentially modulated in older and younger systemic leaves of Solanum lycopersicum
 
Creator Kundu, Anish
 Mishra, Shruti
Vadassery, Jyothilakshmi
 
Subject Solanum lycopersicum
Spodoptera litura
Direct defense
Metabolite profling 
Primary metabolite
Lignin
Chlorogenic acid
 
Description Accepted date: 4 July 2018
Main conclusion: Metabolite profling, biochemical assays, and transcript analysis revealed diferential modulation
of specifc induced defense responses in local, older, and younger systemic leaves in Solanum lycopersicum upon Spodoptera
litura herbivory.
Plants reconfgure their metabolome upon herbivory to induce production of defense metabolites involved in both direct and
indirect defenses against insect herbivores. Herbivory mediated leaf-to-leaf systemic induction pattern of primary and nonvolatile
secondary metabolites is not well studied in tomato. Here, we show that, in cultivated tomato Solanum lycopersicum
herbivory by generalist insect, Spodoptera litura results in diferential alteration of primary metabolites, majorly sugars and
amino acids and specifc secondary metabolites in local, younger, and older systemic leaves. Cluster analysis of 55 metabolites
identifed by GC–MS showed correlation between local and younger systemic leaves. Re-allocation of primary metabolites
like glucose and amino acids from the local to systemic leaf was observed. Secondary metabolites chlorogenic acid, cafeic
acid, and catechin were signifcantly induced during herbivory in systemic leaves. Among specifc secondary metabolites,
chlorogenic acid and catechin signifcantly inhibits S. litura larval growth in all stages. Local leaf exhibited increased lignin
accumulation upon herbivory. Diferential alteration of induced defense responses like reactive oxygen species, polyphenol
oxidase activity, proteinase inhibitor, cell wall metabolites, and lignin accumulation was observed in systemic leaves. The
metabolite alteration also resulted in increased defense in systemic leaves. Thus, comparative analysis of metabolites in local
and systemic leaves of tomato revealed a constant re-allocation of primary metabolites to systemic leaves and diferential
induction of secondary metabolites and induced defenses upon herbivory.
We acknowledge Department of Biotechnology
(DBT), India, through NIPGR Core Grant and BIOCARE Grant, and
Max Planck partner group program of the Max Planck Society (Germany)
for funding this work. We also acknowledge National Bureau
of Agricultural Insect Resources, Bangalore for the initial batch of S.
litura eggs (National Accession No. is: NBAII-MP-NOC-02), Pradeep
Kumar Maurya (NIPGR) for rearing Spodoptera, NIPGR central instrumentation
and phytotron facility, JNU advanced instrumentation facility
for mass spectrometry, and DBT-eLibrary Consortium (DeLCON)
for providing access to e-resources.
 
Date 2018-07-12T09:12:46Z
2018-07-12T09:12:46Z
2018
 
Type Article
 
Identifier Planta, 248(4): 981-997
1432-2048
http://223.31.159.10:8080/jspui/handle/123456789/874
https://link.springer.com/article/10.1007%2Fs00425-018-2953-3
https://doi.org/10.1007/s00425-018-2953-3
 
Language en_US
 
Format application/pdf
 
Publisher Springer Nature