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An innovative root inoculation method to study Ralstonia solanacearum pathogenicity in tomato seedlings

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Title An innovative root inoculation method to study Ralstonia solanacearum pathogenicity in tomato seedlings
 
Creator Singh, N.
Phukan, T.
Sharma, P.L.
Kabyashree, K.
Barman, A.
Kumar, R.
Sonti, Ramesh V.
Genin, S.
Ray, S.K.
 
Subject Ralstonia solanacearum
Tomato Seedlings
Root Inoculation
Pathogenicity
 
Description Accepted date: 13 Nov 2017
In this study, we report Ralstonia solanacearum pathogenicity in the early stages of tomato seedlings by an innovative root inoculation method. Pathogenicity assays were performed under gnotobiotic conditions in microfuge tubes by employing only 6- to 7-day-old tomato seedlings for root inoculation. Tomato seedlings inoculated by this method exhibited the wilted symptom within 48 h and the virulence assay can be completed in 2 weeks. Colonization of the wilted seedlings by R. solanacearum was confirmed by using gus staining as well as fluorescence microscopy. Using this method, mutants in different virulence genes such as hrpB, phcA, and pilT could be clearly distinguished from wild-type R. solanacearum. The method described here is economic in terms of space, labor, and cost as well as the required quantity of bacterial inoculum. Thus, the newly developed assay is an easy and useful approach for investigating virulence functions of the pathogen at the seedling stage of hosts, and infection under these conditions appears to require pathogenicity mechanisms used by the pathogen for infection of adult plants.
N. Singh thanks the Department of Biotechnology (DBT), Government of India (GoI) for the fellowship (DBT-JRF/SRF). A. Barman and P. L. Sharma thank the DBT and UExcel grant for post doc and senior research fellowships, respectively. T. Phukan thanks the University Grants Commission, GoI, for the BSR fellowship. K. Kabyashree thanks UGC for the NET-JRF fellowship. S. K. Ray, K. Kabyashree, and R. V. Sonti thank DBT, GoI for the research grant under the twinning project (BT/301/NE/TBP/2012). S. K. Ray, A. Barman, and S. Genin thank CEFIPRA for the Indo-French project grant (4800-B1). S. K. Ray’s lab research is also supported by various departmental projects such as UGC-SAP (DSR II), DST-FIST, and DBT-Strengthening NE.
 
Date 2018-04-03T06:39:44Z
2018-04-03T06:39:44Z
2018
 
Type Article
 
Identifier Phytopathology, 108(4): 436-442
1943-7684
http://223.31.159.10:8080/jspui/handle/123456789/844
https://apsjournals.apsnet.org/doi/10.1094/PHYTO-08-17-0291-R
https://doi.org/10.1094/PHYTO-08-17-0291-R
 
Language en_US
 
Format application/pdf
 
Publisher American Phytopathological Society