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Whole mount in situ localization of miRNAs and target mRNA transcripts in plants

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Title Whole mount in situ localization of miRNAs and target mRNA transcripts in plants
 
Creator Gautam, Vibhav
Singh, Archita
Verma, Swati
Singh, Sharmila
Chatterjee, Sourav
Sarkar, Ananda K.
 
Subject In situ
miRNA
Gene expression
Arabidopsis
Root development
 
Description Accepted date: 03 April 2019
The functional characterization of miRNAs often involves understanding of their spatiotemporal expression, which mostly relies on reporter-based or in situ hybridization studies. The available in situ localization methods follow separate protocols for pre-hybridization, hybridization, post-hybridization, and detection steps for both miRNA and mRNA transcripts in plants. In this study, we present a single method which can be used for whole mount in situ localization of both miRNAs and mRNAs in different plant tissues. Our modified method provides enhanced sensitivity for the localization of miRNA and their target transcripts. Consequently, a less laborious, time-saving, economic and efficient method has been proposed by the modification of pre-hybridization, hybridization, post-hybridization and detection steps.
V.G., Ar.S., S.C. and S.S. thank Council for Scientific and Industrial Research (CSIR) and Department of Biotechnology, Ministry of Science and Technology (DBT project# BT/PR12766/BPA/118/63/2015), India, and National Institute of Plant Genome Research (NIPGR), New Delhi, India, for funding and internal Grants. S.V. thanks Department of Science and Technology (DST)-Science and Engineering Research Board (SERB) National-PostDoctoral Fellowship (N-PDF grant no. PDF/2016/002423). A.S. thanks NIPGR, New Delhi, India, for fellowship and grants.
 
Date 2019-05-16T11:22:48Z
2019-05-16T11:22:48Z
2019
 
Type Article
 
Identifier 3 Biotech 9(5): 193
2190-5738
http://223.31.159.10:8080/jspui/handle/123456789/949
https://link.springer.com/article/10.1007%2Fs13205-019-1704-x
https://doi.org/10.1007/s13205-019-1704-x
 
Language en_US
 
Publisher Springer Nature Publishing AG