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Transcriptome profiling illustrates expression signatures of dehydration tolerance in developing grasspea seedlings

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Title Transcriptome profiling illustrates expression signatures of dehydration tolerance in developing grasspea seedlings
 
Creator Rathi, Divya
Gayali, Saurabh
Pareek, Akanksha
Chakraborty, Subhra
Chakraborty, Niranjan
 
Subject Grasspea
Transcript diversity
Metabolome profling
Water-defcit
Stress response
Biomarkers
 
Description Accepted date: 21 December 2018
Main conclusion This study highlights dehydration-mediated temporal changes in physicochemical, transcriptome
and metabolome profles indicating altered gene expression and metabolic shifts, underlying endurance and adaptation to stress tolerance in the marginalized crop, grasspea.
Grasspea, often regarded as an orphan legume, is recognized to be fairly tolerant to water-defcit stress. In the present study,
3-week-old grasspea seedlings were subjected to dehydration by withholding water over a period of 144 h. While there
were no detectable phenotypic changes in the seedlings till 48 h, the symptoms appeared during 72 h and aggravated upon
prolonged dehydration. The physiological responses to water-defcit stress during 72–96 h displayed a decrease in pigments,
disruption in membrane integrity and osmotic imbalance. We evaluated the temporal efects of dehydration at the transcriptome and metabolome levels. In total, 5201 genes of various functional classes including transcription factors, cytoplasmic
enzymes and structural cell wall proteins, among others, were found to be dehydration-responsive. Further, metabolome profling revealed 59 dehydration-responsive metabolites including sugar alcohols and amino acids. Despite the lack of genome
information of grasspea, the time course of physicochemical and molecular responses suggest a synchronized dehydration
response. The cross-species comparison of the transcriptomes and metabolomes with other legumes provides evidence for
marked molecular diversity. We propose a hypothetical model that highlights novel biomarkers and explain their relevance
in dehydration-response, which would facilitate targeted breeding and aid in commencing crop improvement eforts.
This work was supported by Grants (38/1385/14/EMR-II)
from the Council of Scientifc and Industrial Research (CSIR), Govt.
of India. We also thank the CSIR for providing predoctoral fellowship
to DR and SG as well as University Grants Commission (UGC) for
providing predoctoral fellowship to AP. We express our sincere gratitude to Tatyana Goldberg, Technical University of Munich, Germany, for helping with the
localization prediction.
 
Date 2019-01-15T11:00:40Z
2019-01-15T11:00:40Z
2019
 
Type Article
 
Identifier Planta, 250(3): 839-855
1432-2048
http://223.31.159.10:8080/jspui/handle/123456789/913
https://link.springer.com/article/10.1007%2Fs00425-018-03082-2
https://doi.org/10.1007/s00425-018-03082-2
 
Language en_US
 
Format application/pdf
 
Publisher Springer Nature