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Bacteria-fungal confrontation and fungal growth prevention assay

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Title Bacteria-fungal confrontation and fungal growth prevention assay
 
Creator Kumar, Rahul
Swain, Durga Madhab
Yadav, Sunil Kumar
Tyagi, Isha
Kumar, Rajeev
Das, Joyati
Ghosh, Srayan
Jha, Gopaljee
 
Subject Sheath blight disease
Rhizoctonia solani
Burkholderia gladioli
Bacterial mycophagy
Bacterial-fungal interaction
 
Description Accepted date: 29 Dec 2017
There are some bacteria which can grow and multiply at the cost of living fungal biomass. They can potentially utilize fungi as a source of nutrients to forage over them. Such phenomenon is known as bacterial mycophagy, however, its mechanistic insights need to be explored to identify the molecules involved in mycophagy for potential utilization in controlling various fungal diseases. Recently we have demonstrated that a rice-associated bacteria Burkholderia gladioli strain NGJ1 exhibits mycophagous ability on several fungi, including Rhizoctonia solani, the necrotrophic fungal pathogen causing sheath blight disease in rice. We hereby describe our validated and efficient methods used to study B. gladioli strain NGJ1-R. solani interactions. These methodologies would be useful for designing assays to study the confrontation between bacteria and fungi which in turn enable discovery of novel antifungal molecules from such bacteria.
The research funding from DBT, Government of India and core research grant from National Institute of Plant Genome Research, India to support the GJ lab are gratefully acknowledged. RK and SG acknowledge fellowship from CSIR and IT, SKY, JD acknowledges fellowship from DBT, Govt. of India. The authors declare no conflict of interest.
 
Date 2018-02-06T06:49:58Z
2018-02-06T06:49:58Z
2018
 
Type Article
 
Identifier Bio-Protocol 8(2): e2694
2331-8325
http://223.31.159.10:8080/jspui/handle/123456789/834
https://bio-protocol.org/e2694#biaoti0
10.21769/BioProtoc.2694
 
Language en_US
 
Format application/pdf
 
Publisher Bio-protocol LLC.