Bacteria-fungal confrontation and fungal growth prevention assay
NIPGR Digital Knowledge Repository (NDKR)
View Archive InfoField | Value | |
Title |
Bacteria-fungal confrontation and fungal growth prevention assay
|
|
Creator |
Kumar, Rahul
Swain, Durga Madhab Yadav, Sunil Kumar Tyagi, Isha Kumar, Rajeev Das, Joyati Ghosh, Srayan Jha, Gopaljee |
|
Subject |
Sheath blight disease
Rhizoctonia solani Burkholderia gladioli Bacterial mycophagy Bacterial-fungal interaction |
|
Description |
Accepted date: 29 Dec 2017
There are some bacteria which can grow and multiply at the cost of living fungal biomass. They can potentially utilize fungi as a source of nutrients to forage over them. Such phenomenon is known as bacterial mycophagy, however, its mechanistic insights need to be explored to identify the molecules involved in mycophagy for potential utilization in controlling various fungal diseases. Recently we have demonstrated that a rice-associated bacteria Burkholderia gladioli strain NGJ1 exhibits mycophagous ability on several fungi, including Rhizoctonia solani, the necrotrophic fungal pathogen causing sheath blight disease in rice. We hereby describe our validated and efficient methods used to study B. gladioli strain NGJ1-R. solani interactions. These methodologies would be useful for designing assays to study the confrontation between bacteria and fungi which in turn enable discovery of novel antifungal molecules from such bacteria. The research funding from DBT, Government of India and core research grant from National Institute of Plant Genome Research, India to support the GJ lab are gratefully acknowledged. RK and SG acknowledge fellowship from CSIR and IT, SKY, JD acknowledges fellowship from DBT, Govt. of India. The authors declare no conflict of interest. |
|
Date |
2018-02-06T06:49:58Z
2018-02-06T06:49:58Z 2018 |
|
Type |
Article
|
|
Identifier |
Bio-Protocol 8(2): e2694
2331-8325 http://223.31.159.10:8080/jspui/handle/123456789/834 https://bio-protocol.org/e2694#biaoti0 10.21769/BioProtoc.2694 |
|
Language |
en_US
|
|
Format |
application/pdf
|
|
Publisher |
Bio-protocol LLC.
|
|