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Methods for measuring nitrate reductase, nitrite levels, and nitric oxide from plant tissues

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Title Methods for measuring nitrate reductase, nitrite levels, and nitric oxide from plant tissues
 
Creator Wany, Aakanksha
Pathak, Pradeep Kumar
Gupta, Kapuganti Jagadis
 
Subject DAF-FM
Nitrate
Nitrate reductase
Nitrite
Nitric oxide
 
Description Accepted date: 09 October 2019
Nitrogen (N) is one of the most important nutrients which exist in both inorganic and organic forms. Plants assimilate inorganic form of N [nitrate (NO3−), nitrite (NO2−) or ammonium (NH4+)] and incorporate into amino acids. The metabolism of N involves a series of events such as sensing, uptake, and assimilation. The initial stage is sensing, triggered by nitrate or ammonium signals initiating signal transduction processes in N metabolism. The assimilation pathway initiates with NO3−/NH4+ transport to roots via specific high and low affinity (HATs and LATs) nitrate transporters or directly via ammonium transporters (AMTs). In cytosol the NO3− is reduced to NO2− by cytosolic nitrate reductase (NR) and the produced NO2− is further reduced to NH4+ by nitrite reductase (NiR) in plastids. NR has capability to reduce NO2− to nitric oxide (NO) under specific conditions such as hypoxia, low pH, and pathogen infection. The produced NO acts as a signal for wide range of processes such as plant growth development and stress. Here, we provide methods to measure NR activity, NO2− levels, and NO production in plant tissues.
 
Date 2019-10-15T07:54:26Z
2019-10-15T07:54:26Z
2020
 
Type Book chapter
 
Identifier Methods in Molecular Biology, 2057: 15-26
978-1-4939-9790-9
http://223.31.159.10:8080/jspui/handle/123456789/1003
https://link.springer.com/protocol/10.1007/978-1-4939-9790-9_2
https://doi.org/10.1007/978-1-4939-9790-9_2
 
Language en_US
 
Format application/pdf
 
Publisher Springer Nature Publishing AG