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Stress inducible galactinol synthase of chickpea (CaGolS) implicates in heat and oxidative stress tolerance through reducing stress induced excessive reactive oxygen species accumulation

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Title Stress inducible galactinol synthase of chickpea (CaGolS) implicates in heat and oxidative stress tolerance through reducing stress induced excessive reactive oxygen species accumulation
 
Creator Salvi, Prafull
Kamble, Nitin Uttam
Majee, Manoj
 
Subject Environmental and Stress Responses
Abiotic stress
Chickpea
Galactinol
Galactinol Synthase
RFO
ROS
 
Description Accepted date: 02 November 2017
Raffinose Family Oligosaccharides (RFOs) participates in various aspects of plant physiology and galactinol synthase (GolS, EC 2.4.1.123) catalyzes the key step of RFO biosynthesis. Stress induced accumulation of RFOs particularly galactinol and raffinose has been reported in few plants; however their precise role and mechanistic insight in stress adaptation remain elusive. In present study, we have shown that the GolS activity as well as galactinol and raffinose content are significantly increased in response to various abiotic stresses in chickpea. Transcriptional analysis indicated that the CaGolS1 and CaGolS2 genes are induced in response to different abiotic stresses. Interestingly, heat and oxidative stress preferentially induce CaGolS1 over CaGolS2. Insilco analysis revealed several common yet distinct cis-acting regulatory elements in their 5' upstream regulatory sequences. Further, in vitro biochemical analysis revealed that CaGolS1 enzyme functions better in stressful conditions than CaGolS2 enzyme. Finally, Arabidopsis transgenic plants constitutively overexpressing CaGolS1 or CaGolS2 not only exhibits significantly increased galactinol but also raffinose content and display better growth responses than wild type or vector control plants when exposed to heat and oxidative stress. Further, improved tolerance of transgenic lines are associated with reduced accumulation of reactive oxygen species (ROS) and consequent lipid peroxidation as compared to control plants.Collectively, our data implies that GolS enzyme activity and consequent galactinol and raffinose content are significantly increased in response to stresses to mitigate stress induced growth inhibition by restricting excessive ROS accumulation and consequent lipid peroxidation in plants.
This work was supported by the grant (BT/AGR/CG-PhaseII/01/2014) from Department of Biotechnology,
Government of India and NIPGR core grant. P.S. and N.U.K thank Council of Scientific and Industrial Research and University Grant Commission, Government of India, for research fellowship.
 
Date 2017-11-13T06:35:59Z
2017-11-13T06:35:59Z
2018
 
Type Article
 
Identifier Plant and Cell Physiology, 59(1):155-166
1471-9053
http://223.31.159.10:8080/jspui/handle/123456789/797
https://academic.oup.com/pcp/article-lookup/doi/10.1093/pcp/pcx170
https://doi.org/10.1093/pcp/pcx170
 
Language en_US
 
Format application/pdf
 
Publisher Oxford University Press