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Production and characterization of polyhydroxyalkanoate from lignin derivatives by Pandoraea sp. ISTKB

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Title Production and characterization of polyhydroxyalkanoate from lignin derivatives by Pandoraea sp. ISTKB
 
Creator Kumar, Madan
Singhal, Anjali
Verma, Praveen Kumar
Thakur, Indu Shekhar
 
Subject Pharmacology
Nucleic acids
Nucleic acid structure
Genomics
Genetics
 
Description Accepted date: 8 November 2017
The present study investigates polyhydroxyalkanoate (PHA) production from lignin and its derivatives by a previously reported lignin-degrading bacterial strain Pandoraea sp. ISTKB. PHA production was screened by fluorescence microscopy and flow cytometry using a Nile red stain. PHA and biomass accumulation, while screening, was found to be maximum on 4-hydroxybenzoic acid followed by p-coumaric acid, vanillic acid, 2,6-dimethoxyphenol, and kraft lignin after 96 h. Monomer composition was analyzed by gas chromatography–mass spectrometry (GC–MS) and was followed by Fourier transform infrared and 1H NMR analysis, indicating PHA to be a copolymer of P(hydroxybutyrate-co-hydroxyvalerate). Genomic analysis of Pandoraea sp. ISTKB also complemented the results of GC–MS and NMR, and the relevant genes responsible for the synthesis of small chain length PHA were discovered in the genome. Process parameters were optimized by response surface methodology for enhanced production of PHA and biomass on 4-hydroxybenzoate. Optimization results showed 30 and 66% increase in the biomass and PHA production, respectively. The results obtained were promising and indicated that if lignin is depolymerized into low-molecular-weight intermediates, then it can easily be utilized and converted into value-added products like PHA by microbes.
We thank Department of Biotechnology (DBT), Ministry of
Science and Technology, Government of India, for supporting
research work. The authors thank Council of Scientific and
Industrial Research (CSIR), New Delhi, India, and University
Grants Commission (UGC), New Delhi, Government of India,
for providing Research Fellowship. We thank Advanced
Instrumentation Research Facility for FACS, GC−MS, TEM,
NMR, FTIR and microscopy and lab members for their help
during experiments.
 
Date 2018-02-20T08:47:57Z
2018-02-20T08:47:57Z
2017
 
Type Article
 
Identifier ACS Omega, 2(12): 9156-9163
2470-1343
http://223.31.159.10:8080/jspui/handle/123456789/838
https://pubs.acs.org/doi/10.1021/acsomega.7b01615
10.1021/acsomega.7b01615
 
Language en_US
 
Format application/pdf
 
Publisher American Chemical Society