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Nitric oxide alters nitrogen metabolism and PIN gene expressions by playing protective role in arsenic challenged Brassica juncea L.

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Title Nitric oxide alters nitrogen metabolism and PIN gene expressions by playing protective role in arsenic challenged Brassica juncea L.
 
Creator Praveen, Afsana
Pandey, Ashutosh
Gupta, Meetu
 
Subject Nitric oxide
Arsenite
Oxidative stress
B.juncea
N pathway
Auxin transporter
 
Description Accepted dated: 13 March 2019
Plants have ability to adapt themselves through altering their growth process. In the present study, we examined exogenous application of nitric oxide (NO) on nitrogen metabolism and auxin (PIN) gene expression, and its possible role in alleviation of arsenic (As) toxicity in Brassica juncea seedlings. Seven days old hydroponically grown B. juncea seedlings were exposed to AsIII (150 μM), Sodium nitroprusside (NO donor, 100 μM), AsIII + SNP and control (without metal)for 48 h. Experimental results revealed that AsIII stress: enhanced the level of nitrite, NiR activity, NO3− and NH4+content as well as NADH-GOGAT activity; but GDH level decreased; enhanced content of amino acids; upregulated gene expression level of N metabolism and downregulated polar auxin transporter genes (PIN); inhibited plant growth and morphological parameters; increased MDA, H2O2, cysteine, proline content, enzymatic antioxidants (SOD, CAT, APX; GSH, TT, NPT); and decreased nutrient content. AsIII + SNP combination reduced the accumulation of As; improved growth; chlorophyll, protein and mineral nutrient content by scavenging ROS generation; maintained amino acids content; downregulated expression of N metabolism genes and upregulated expression of auxin transporter (PIN) genes . Additional biochemical data depicts reduction in the level of nitrogen related enzymatic activities, and other stress related parameters. Overall, this study provides an integrated view that exogenous SNP (NO donor) supplementation alleviated the inhibitory role of AsIII in B. juncea seedlings by altering nutrients, amino acids and auxin redistribution via expression of nitrogen and PIN gene profiling.
AP(DBT/JRF/14/AL/250) thanks Department of Biotechnology
(DBT), Govt. of India for fellowship.
 
Date 2019-04-05T07:14:57Z
2019-04-05T07:14:57Z
2019
 
Type Article
 
Identifier Ecotoxicology and Environmental Safety, 176: 95-107
0147-6513
http://223.31.159.10:8080/jspui/handle/123456789/939
https://www.sciencedirect.com/science/article/pii/S014765131930315X?via%3Dihub
https://doi.org/10.1016/j.ecoenv.2019.03.054
 
Language en_US
 
Format application/pdf
 
Publisher Elsevier B.V.