Record Details

Carboxylate clamp tetratricopeptide repeat (TPR) domain containing Hsp90 cochaperones in Triticeace: an insight into structural and functional diversification

NIPGR Digital Knowledge Repository (NDKR)

View Archive Info
 
 
Field Value
 
Title Carboxylate clamp tetratricopeptide repeat (TPR) domain containing Hsp90 cochaperones in Triticeace: an insight into structural and functional diversification
 
Creator Mishra, Divya
Shekhar, Shubhendu
Chakraborty, Subhra
Chakraborty, Niranjan
 
Subject Bread wheat
Cochaperone
Heterologous expression
Stress tolerance
Tetratricopeptide repeat
Triticeace
 
Description Accepted date: 16 June 2018
The molecular chaperones serve as surveillance molecules that mediates regulatory crosstalk between protein folding and degradation pathways under natural and stress conditions. In present study, we focused on the diversification and role of tetratricopeptide repeat (TPR) domain containing Hsp90 cochaperones. These cochaperone were recognized by the presence of three motifs of TPR with the basic conserved residues often referred to as carboxylate clamp (CC). A total of 213 putative CC-TPRs were found in Triticeace, clustered into 16 groups, amongst which few CC-TPR families such as TPR-RPAP3 and TPR-SMYD were documented. Domain architecture and genomic organization revealed that CC-TPRs are very diverse in nature. Evolutionary analyses showed that CC-TPRs are conserved, stable and ubiquitous in nature. Analysis of available RNA-seq data revealed a high degree of tissue-specific expression of 1-TPR and TaTPR-FKBP family members at various developmental stages. The transcripts of TaCC-TPRs displayed differential expression in two contrasting wheat cultivars under abiotic stress conditions. Complementation and heterologous expression of TaTPR-FKBP5 in yeast conferred abiotic stress tolerance. Together, these results provide a glimpse into the genetic diversity and evolution of CC-TPRs in Triticeace, which would help to better understand of how TPR-domain cochaperones function in plants.
This work was supported by the National Institute of Plant Genome Research, New Delhi, India. We kindly acknowledge the University Grant Commission (UGC), Govt. of India for providing predoctoral fellowship to D.M. and DST-SERB for providing postdoctoral fellowship to S.S. Assistance of Mr. Shankar Acharya for computational work is greatly acknowledged.
 
Date 2018-07-10T05:42:25Z
2018-07-10T05:42:25Z
2018
 
Type Article
 
Identifier Environmental and Experimental Botany, 155: 31-44
0098-8472
http://223.31.159.10:8080/jspui/handle/123456789/869
https://www.sciencedirect.com/science/article/pii/S0098847218303939
https://doi.org/10.1016/j.envexpbot.2018.06.020
 
Language en_US
 
Format application/pdf
 
Publisher Elsevier B.V.