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Dehydration-responsive nuclear proteome landscape of chickpea (Cicer arietinum L.) reveals phosphorylation-mediated regulation of stress response

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Title Dehydration-responsive nuclear proteome landscape of chickpea (Cicer arietinum L.) reveals phosphorylation-mediated regulation of stress response
 
Creator Barua, Pragya
Lande, Nilesh Vikram
Subba, Pratigya
Gayen, Dipak
Pinto, Sneha
Prasad, T.S. Keshav
Chakraborty, Subhra
Chakraborty, Niranjan
 
Subject alternative splicing
cellular signalling
dehydration
legume
nuclear proteome
nuclear phosphoproteome
stress tolerance
 
Description Accepted date: 30 April 2018
Non-availability of water or dehydration remains recurring climatic disorder affecting yield of major food crops, legumes in particular. Nuclear proteins (NP) and phosphoproteins (NPPs) execute crucial cellular functions that form the regulatory hub for coordinated stress response. Phosphoproteins hold enormous influence over cellular signalling. Four-week-old seedlings of a grain legume, chickpea, were subjected to gradual dehydration and nuclear proteins were extracted from unstressed control as well as from 72 and 144 h stressed tissues. We identified 4832 NPs and 478 phosphosites, corresponding to 299 unique NPPs involved in multivariate cellular processes including protein modification and gene expression regulation, among others. The identified proteins included several novel kinases, phosphatases and transcription factors, besides 660 uncharacterised proteins. Spliceosome complex and splicing related proteins were dominant among differentially regulated NPPs, indicating their dehydration modulated regulation. Phospho-motif analysis revealed stress-induced enrichment of proline-directed serine phosphorylation. Association mapping of NPPs revealed predominance of differential phosphorylation of spliceosome and splicing associated proteins. Also, regulatory proteins of key processes viz., protein degradation, regulation of flowering time and circadian clock were observed to undergo dehydration-induced dephosphorylation. The characterization of novel regulatory proteins would provide new insights into stress adaptation and enable directed genetic manipulations for developing climate-resilient crops.
This work was supported by a grant [BT/AGR/CG-Phase-II] from the Department of
Biotechnology (DBT), Govt. of India and DST-SERB [EMR/2015/001870]. The authors
thank DBT, Council of Scientific and Industrial Research (CSIR) and DST-SERB for
providing research fellowship to PB, NVL and DG respectively. The authors gratefully
acknowledge International Crops Research Institute for the Semi-Arid Tropics (ICRISAT),
Hyderabad, India for providing chickpea seeds.
 
Date 2018-05-14T11:51:50Z
2018-05-14T11:51:50Z
2019
 
Type Article
 
Identifier Plant, Cell & Environment, 42(1): 230-244
1365-3040
http://223.31.159.10:8080/jspui/handle/123456789/856
https://onlinelibrary.wiley.com/doi/abs/10.1111/pce.13334
https://doi.org/10.1111/pce.13334
 
Language en_US
 
Format application/pdf
 
Publisher John Wiley & Sons