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Glucose-regulated HLP1 acts as a key molecule in governing thermomemory

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Title Glucose-regulated HLP1 acts as a key molecule in governing thermomemory
 
Creator Sharma, Mohan
Banday, Zeeshan Zahoor
Shukla, Brihaspati N.
Laxmi, Ashverya
 
Subject thermomemory
HLP1
Glucose-regulated
 
Description Accepted: 4 March 2019
Induction of heat shock proteins (HSPs) in response to heat stress (HS) is indispensable for conferring thermotolerance. Glucose, a fundamental signaling and metabolic molecule, provides energy to stressed seedlings to combat stress. The recovery of stressed plants from detrimental HS in response to glucose is largely mediated by HSPs, but the mechanistic basis of this thermotolerance is not well defined. In this study, we show that glucose has a prominent role in providing thermotolerance. Glucose-mediated thermotolerance involves HSP induction via the TOR-E2Fa signaling module. Apart from HSPs, TOR-E2Fa also regulates the Arabidopsis (Arabidopsis thaliana) ortholog of human Hikeshi, named HIKESHI-LIKE PROTEIN1 (HLP1). Expression of proHLP1::GUS in the shoot apical meristem (SAM) after heat stress coincides with TOR-E2Fa expression, substantiating a role for TOR-E2Fa-HLP1 in providing thermotolerance. We also demonstrate that glucose along with heat could induce proliferation activity in the SAM after HS recovery, which was arrested by the TOR inhibitor AZD-8055. Molecular and physiological studies suggest that HS-activated HSFA1s also positively regulate HLP1 transcription, suggesting convergence of the glucose and HS signaling pathways. Loss of functional HLP1 causes HS hypersensitivity, while HLP1 overexpressors (OEs) display increased thermotolerance. HLP1 binds to the promoters of glucose-regulated HS responsive genes and promotes chromatin acetylation. In addition, glucose modifies the chromatin landscape at thermomemory-related loci by promoting H3K4 trimethylation (H3K4me3). Glucose-primed accumulation of H3K4me3 at thermomemory-associated loci is mediated through HLP1. These findings reveal the novel function of glucose-regulated HLP1 in mediating thermotolerance/thermomemory response.
M.S. and A.L. conceived and designed the experiments. M.S. performed all the experiments.
M.S. and Z.Z.B. performed ChIP experiments, localization, complementation and
bioinformatics studies. M.S. and B.N.S. performed seedling survival assays, chlorophyll
estimation and immuno-blotting experiments. B.N.S. provided technical assistance to M.S.
throughout the experiments. M.S. and A.L. analysed the data. M.S wrote the manuscript with
input from Z.Z.B. A.L. supervised and complemented the writing.
This work was supported by a core grant from the National Institute of Plant Genome
Research, New Delhi, India to A.L. M.S. received a fellowship from the Department of
Biotechnology and Z.Z.B received a fellowship from the Department of Biotechnology, India
under project # BT/PR8001/BRB/10/1211/2013.
 
Date 2019-03-26T09:47:37Z
2019-03-26T09:47:37Z
2019
 
Type Article
 
Identifier Plant Physiology, 180(2): 1081-1100
1532-2548
http://223.31.159.10:8080/jspui/handle/123456789/933
http://www.plantphysiol.org/content/early/2019/03/19/pp.18.01371.long
https://doi.org/10.1104/pp.18.01371
 
Language en_US
 
Format application/pdf
 
Publisher American Society of Plant Biologists